13C-NMR experiments were performed on photo-excited fully and partially 13C-labelled LOV2 domains of the blue-light receptor phototropin. In the present paper, we report on nuclear-spin polarized tryptophan resonances that are generated by light-induced intraprotein electron transfer to the FMN cofactor. The spectra are discussed with respect to earlier data obtained from 13C-NMR experiments on unlabelled LOV2 domains that have been reconstituted with FMN 13C isotopologues.

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