Nucleic acids are routinely and readily analysed using the A260/A280 ratio, although this method is known to be prone to erroneous results owing to contaminants in solution that absorb at similar wavelengths. The aim of the present review, while highlighting the problems and alternatives of using UV spectrophotometry for nucleic acid measurements, is to bring forth an observational result from our recent studies, namely that DO (dissolved oxygen) and nitrogen can alter the A260 of aqueous DNA solutions. Our finding is of importance because DO is highly variable between protocols and storage conditions of DNA preparations. The physicochemical nature of the oxygen–DNA interactions is briefly discussed.
Dissolved oxygen alteration of the spectrophotometric analysis and quantification of nucleic acid solutions
- Views Icon Views
- Share Icon Share
- Cite Icon Cite
Rupak Doshi, Philip J.R. Day, Nicola Tirelli; Dissolved oxygen alteration of the spectrophotometric analysis and quantification of nucleic acid solutions. Biochem Soc Trans 1 April 2009; 37 (2): 466–470. doi: https://doi.org/10.1042/BST0370466
Download citation file: