Most temperate phages encode an integrase for integration and excision of the prophage. Integrases belong either to the λ Int family of tyrosine recombinases or to a subgroup of the serine recombinases, the large serine recombinases. Integration by purified serine integrases occurs efficiently in vitro in the presence of their cognate (~50 bp) phage and host attachment sites, attP and attB respectively. Serine integrases require an accessory protein, Xis, to promote excision, a reaction in which the products of the integration reaction, attL and attR, recombine to regenerate attP and attB. Unlike other directional recombinases, serine integrases are not controlled by proteins occupying accessory DNA-binding sites. Instead, it is thought that different integrase conformations, induced by binding to the DNA substrates, control protein–protein interactions, which in turn determine whether recombination proceeds. The present review brings together the evidence for this model derived from the studies on φC31 integrase, Bxb1 integrase and other related proteins.
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April 2010
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Conference Article|
March 22 2010
Site-specific recombination by φC31 integrase and other large serine recombinases
Margaret C.M. Smith;
Margaret C.M. Smith
1
*Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, U.K.
1To whom correspondence should be addressed (email [email protected]).
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William R.A. Brown;
William R.A. Brown
†Institute of Genetics, University of Nottingham, Nottingham NG7 2UH, U.K.
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Andrew R. McEwan;
Andrew R. McEwan
*Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, U.K.
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Paul A. Rowley
Paul A. Rowley
*Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, U.K.
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Publisher: Portland Press Ltd
Received:
July 24 2009
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2010 Biochemical Society
2010
Biochem Soc Trans (2010) 38 (2): 388–394.
Article history
Received:
July 24 2009
Citation
Margaret C.M. Smith, William R.A. Brown, Andrew R. McEwan, Paul A. Rowley; Site-specific recombination by φC31 integrase and other large serine recombinases. Biochem Soc Trans 1 April 2010; 38 (2): 388–394. doi: https://doi.org/10.1042/BST0380388
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