NMR spectroscopy was used to explore the different aspects of the normal and pathological functions of tau, but proved challenging because the protein contains 441 amino acids and has poor signal dispersion. We have set out to dissect the phosphorylation patterns of tau in order to understand better its role in the aggregation process and microtubule-binding regulation. Our current knowledge on the functional consequences of specific phosphorylations is still limited, mainly because producing and assessing quantitatively phosphorylated tau samples is far from straightforward, even in vitro. We use NMR spectroscopy as a proteomics tool to characterize the phosphorylation patterns of tau, after in vitro phosphorylation by recombinant kinases. The phosphorylated tau can next be use for functional assays or interaction assays with phospho-dependent protein partners, such as the prolyl cis–trans isomerase Pin1.
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August 2010
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Conference Article|
July 26 2010
NMR spectroscopy of the neuronal tau protein: normal function and implication in Alzheimer's disease
Isabelle Landrieu;
Isabelle Landrieu
1
*CNRS-UMR 8576 - IFR 147, Lille 1 Science and Technology University, 59655 Villeneuve d'Ascq Cedex, France
1To whom correspondence should be addressed (email isabelle.landrieu@univ-lille1.fr).
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Arnaud Leroy;
Arnaud Leroy
†Laboratoire de biochimie appliquée, University Paris XI, Faculty of pharmacy, Chatenay-Malabry Cedex, France
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Caroline Smet-Nocca;
Caroline Smet-Nocca
*CNRS-UMR 8576 - IFR 147, Lille 1 Science and Technology University, 59655 Villeneuve d'Ascq Cedex, France
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Isabelle Huvent;
Isabelle Huvent
*CNRS-UMR 8576 - IFR 147, Lille 1 Science and Technology University, 59655 Villeneuve d'Ascq Cedex, France
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Laziza Amniai;
Laziza Amniai
*CNRS-UMR 8576 - IFR 147, Lille 1 Science and Technology University, 59655 Villeneuve d'Ascq Cedex, France
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Malika Hamdane;
Malika Hamdane
‡INSERM U815, Lille 2 University, School of Medicine, 59000 Lille, France
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Nathalie Sibille;
Nathalie Sibille
*CNRS-UMR 8576 - IFR 147, Lille 1 Science and Technology University, 59655 Villeneuve d'Ascq Cedex, France
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Luc Buée;
Luc Buée
‡INSERM U815, Lille 2 University, School of Medicine, 59000 Lille, France
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Jean-Michel Wieruszeski;
Jean-Michel Wieruszeski
*CNRS-UMR 8576 - IFR 147, Lille 1 Science and Technology University, 59655 Villeneuve d'Ascq Cedex, France
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Guy Lippens
Guy Lippens
*CNRS-UMR 8576 - IFR 147, Lille 1 Science and Technology University, 59655 Villeneuve d'Ascq Cedex, France
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Publisher: Portland Press Ltd
Received:
January 05 2010
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2010 Biochemical Society
2010
Biochem Soc Trans (2010) 38 (4): 1006–1011.
Article history
Received:
January 05 2010
Citation
Isabelle Landrieu, Arnaud Leroy, Caroline Smet-Nocca, Isabelle Huvent, Laziza Amniai, Malika Hamdane, Nathalie Sibille, Luc Buée, Jean-Michel Wieruszeski, Guy Lippens; NMR spectroscopy of the neuronal tau protein: normal function and implication in Alzheimer's disease. Biochem Soc Trans 1 August 2010; 38 (4): 1006–1011. doi: https://doi.org/10.1042/BST0381006
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