The CPC [chromosomal passenger complex; INCENP (inner centromere protein), Aurora B kinase, survivin and borealin] is implicated in many mitotic processes. In the present paper we describe how we generated DT40 conditional-knockout cell lines for incenp1 and survivin1 to better understand the role of these CPC subunits in the control of Aurora B kinase activity. These lines enabled us to reassess current knowledge of survivin function and to show that INCENP acts as a rheostat for Aurora B activity.
Use of DT40 conditional-knockout cell lines to study chromosomal passenger protein function
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Xavier Fant, Kumiko Samejima, Ana Carvalho, Hiromi Ogawa, Zhenjie Xu, Zuojun Yue, William C. Earnshaw, Sandrine Ruchaud; Use of DT40 conditional-knockout cell lines to study chromosomal passenger protein function. Biochem Soc Trans 1 December 2010; 38 (6): 1655–1659. doi: https://doi.org/10.1042/BST0381655
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