The c-di-GMP [bis-(3′–5′)-cyclic dimeric guanosine monophosphate] riboswitch is a macromolecular target in the c-di-GMP second messenger signalling pathway. It regulates many genes related to c-di-GMP metabolism as well as genes involved in bacterial motility, virulence and biofilm formation. The riboswitch makes asymmetric contacts to the bases and phosphate backbone of this symmetric dinucleotide. The phylogenetics suggested and mutagenesis has confirmed that this is a flexible motif where variants can make alternative interactions with each of the guanine bases of c-di-GMP. A mutant riboswitch has been designed that can bind a related molecule, c-di-AMP, confirming the most important contacts made to the ligand. The binding kinetics reveal that this is a kinetically controlled riboswitch and mutations to the riboswitch lead to increases in the off-rate. This riboswitch is therefore flexible in sequence as well as kinetic properties.
Conference Article| March 22 2011
Interactions of the c-di-GMP riboswitch with its second messenger ligand
Kathryn D. Smith;
Scott A. Strobel
Scott A. Strobel 1
*Department of Chemistry, Yale University, 225 Prospect Street, New Haven, CT 06520, U.S.A.
†Department of Molecular Biophysics and Biochemistry, Yale University, 260 Whitney Avenue, New Haven, CT 06520, U.S.A.
1To whom correspondence should be addressed (email firstname.lastname@example.org).
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Kathryn D. Smith, Scott A. Strobel; Interactions of the c-di-GMP riboswitch with its second messenger ligand. Biochem Soc Trans 1 April 2011; 39 (2): 647–651. doi: https://doi.org/10.1042/BST0390647
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