Heterologous multiprotein expression is the tool to answer a number of questions in basic science as well as to convert strains into producers and/or consumers of certain compounds in applied sciences. Multiprotein expression can be driven by plasmids with the disadvantages that the gene dosage might, in some cases, lead to toxic effects and that the continuous addition of antibiotics is undesirable. Stable genomic expression of proteins can forgo these problems and is a helpful and promising tool in synthetic biology. In the present paper, we provide an extract of methods from the toolbox for chromosome-based heterologous expression in Escherichia coli.
Conference Article| November 21 2012
The molecular toolbox for chromosomal heterologous multiprotein expression in Escherichia coli
Johannes Gescher 1
1Institut für Angewandte Biowissenschaften, Angewandte Biologie, Karlsruher Institut für Technologie, Fritz-Haber-Weg 2, D-76131 Karlsruhe, Germany
1To whom correspondence should be addressed (firstname.lastname@example.org).
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Katrin Richter, Johannes Gescher; The molecular toolbox for chromosomal heterologous multiprotein expression in Escherichia coli. Biochem Soc Trans 1 December 2012; 40 (6): 1222–1226. doi: https://doi.org/10.1042/BST20120143
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