Colicins are the only proteins imported by Escherichia coli and thus serve as tools to study the protein import mechanism. Most of the colicins studied degrade DNA, 16S RNA or tRNA in the cytoplasm, or form pores in the cytoplasmic membrane. Two bacteriocins, Cma (colicin M) and Pst (pesticin), affect the murein structure in the periplasm. These two bacteriocins must be imported only across the outer membrane and therefore represent the simplest system for studying protein import. Cma can be reversibly translocated across the outer membrane. Cma and Pst unfold during import. The crystal structure of Pst reveals a phage T4L (T4 lysozyme) fold of the activity domain. Both bacteriocins require energy for import which is translocated from the cytoplasmic membrane into the outer membrane by the Ton system. Cma kills cells only when the periplasmic FkpA PPIase (peptidylprolyl cis–trans isomerase)/chaperone is present.
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Conference Article|
November 21 2012
Import of periplasmic bacteriocins targeting the murein Available to Purchase
Volkmar Braun;
Volkmar Braun
1
1Department of Protein Evolution, Max Planck Institute for Developmental Biology, Spemannstrasse 35, 72076 Tübingen, Germany
1To whom correspondence should be addressed (email[email protected]).
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Stephanie Helbig;
Stephanie Helbig
1Department of Protein Evolution, Max Planck Institute for Developmental Biology, Spemannstrasse 35, 72076 Tübingen, Germany
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Silke I. Patzer
Silke I. Patzer
1Department of Protein Evolution, Max Planck Institute for Developmental Biology, Spemannstrasse 35, 72076 Tübingen, Germany
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Publisher: Portland Press Ltd
Received:
July 12 2012
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2012 The Authors Journal
2012
Biochem Soc Trans (2012) 40 (6): 1449–1455.
Article history
Received:
July 12 2012
Citation
Volkmar Braun, Stephanie Helbig, Silke I. Patzer; Import of periplasmic bacteriocins targeting the murein. Biochem Soc Trans 1 December 2012; 40 (6): 1449–1455. doi: https://doi.org/10.1042/BST20120175
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