Purified phage lysins present an alternative to traditional antibiotics and work by hydrolysing peptidoglycan. Phage lysins have been developed against Gram-positive pathogens such as Bacillus anthracis and Streptococcus pneumoniae, where the peptidoglycan layer is exposed on the cell surface. Addition of the lysin to a bacterial culture results in rapid death of the organism. Gram-negative bacteria are resistant to phage lysins because they contain an outer membrane that protects the peptidoglycan from degradation. We solved crystal structures of a Yersinia pestis outer-membrane protein and the bacteriocin that targets it, which informed engineering of a bacterial–phage hybrid lysin that can be transported across the outer membrane to kill specific Gram-negative bacteria. This work provides a template for engineering phage lysins against a wide variety of bacterial pathogens.
Using a bacteriocin structure to engineer a phage lysin that targets Yersinia pestis
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Petra Lukacik, Travis J. Barnard, Susan K. Buchanan; Using a bacteriocin structure to engineer a phage lysin that targets Yersinia pestis. Biochem Soc Trans 1 December 2012; 40 (6): 1503–1506. doi: https://doi.org/10.1042/BST20120209
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