Although performed by members of all three domains of life, the archaeal version of N-glycosylation remains the least understood. Studies on Haloferax volcanii have, however, begun to correct this situation. A combination of bioinformatics, molecular biology, biochemical and mass spectrometry approaches have served to delineate the Agl pathway responsible for N-glycosylation of the S-layer glycoprotein, a reporter of this post-translational modification in Hfx. volcanii. More recently, differential N-glycosylation of the S-layer glycoprotein as a function of environmental salinity was demonstrated, showing that this post-translational modification serves an adaptive role in Hfx. volcanii. Furthermore, manipulation of the Agl pathway, together with the capability of Hfx. volcanii to N-glycosylate non-native proteins, forms the basis for establishing this species as a glyco-engineering platform. In the present review, these and other recent findings are addressed.
Skip Nav Destination
Article navigation
February 2013
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
Conference Article|
January 29 2013
Add salt, add sugar: N-glycosylation in Haloferax volcanii
Lina Kaminski;
Lina Kaminski
1Department of Life Sciences, Ben Gurion University of the Negev, Beersheva 84105, Israel
Search for other works by this author on:
Shai Naparstek;
Shai Naparstek
1Department of Life Sciences, Ben Gurion University of the Negev, Beersheva 84105, Israel
Search for other works by this author on:
Lina Kandiba;
Lina Kandiba
1Department of Life Sciences, Ben Gurion University of the Negev, Beersheva 84105, Israel
Search for other works by this author on:
Chen Cohen-Rosenzweig;
Chen Cohen-Rosenzweig
1Department of Life Sciences, Ben Gurion University of the Negev, Beersheva 84105, Israel
Search for other works by this author on:
Adi Arbiv;
Adi Arbiv
1Department of Life Sciences, Ben Gurion University of the Negev, Beersheva 84105, Israel
Search for other works by this author on:
Zvia Konrad;
Zvia Konrad
1Department of Life Sciences, Ben Gurion University of the Negev, Beersheva 84105, Israel
Search for other works by this author on:
Jerry Eichler
Jerry Eichler
1
1Department of Life Sciences, Ben Gurion University of the Negev, Beersheva 84105, Israel
1To whom correspondence should be addressed (emailjeichler@bgu.ac.il).
Search for other works by this author on:
Biochem Soc Trans (2013) 41 (1): 432–435.
Article history
Received:
June 07 2012
Citation
Lina Kaminski, Shai Naparstek, Lina Kandiba, Chen Cohen-Rosenzweig, Adi Arbiv, Zvia Konrad, Jerry Eichler; Add salt, add sugar: N-glycosylation in Haloferax volcanii. Biochem Soc Trans 1 February 2013; 41 (1): 432–435. doi: https://doi.org/10.1042/BST20120142
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.