In recent years, HDX-MS (hydrogen–deuterium exchange coupled to MS) on biomolecules has evolved from a niche technique to a powerful method in the investigation of protein dynamics. Protein kinases, in particular, represent an area of active study using this technique owing to their well-characterized protein structures and their relevance to diseases such as cancer, immune disorders and neurodegenerative defects. In the present review, we describe how HDX-MS has revealed important dynamic properties of protein kinases and provided insight into the mechanisms of drug binding.
Conference Article| January 23 2014
HDX-MS takes centre stage at unravelling kinase dynamics
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Kristina Lorenzen, Tony Pawson; HDX-MS takes centre stage at unravelling kinase dynamics. Biochem Soc Trans 1 February 2014; 42 (1): 145–150. doi: https://doi.org/10.1042/BST20130250
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