To suggest and develop intelligent strategies to comprehend the regulation of organelle formation, a deeper mechanistic interpretation requires more than just the involvement of proteins. Our approaches link the formation of endomembranes with both signalling and membrane physical properties. Hitherto, membrane morphology, local physical structure and signalling have not been well integrated. Our studies derive from a cross-disciplinary approach undertaken to determine the molecular mechanisms of nuclear envelope assembly in echinoderm and mammalian cells. Our findings have led to the demonstration of a direct role for phosphoinositides and their derivatives in nuclear membrane formation. We have shown that phosphoinositides and their derivatives, as well as acting as second messengers, are modulators of membrane morphology, and their modifying enzymes regulate nuclear envelope formation. In addition, we have shown that echinoderm eggs can be exploited as a milieu to directly study the roles of phospholipids in maintaining organelle shape. The use of the echinoderm egg is a significant step forward in obtaining direct information about membrane physical properties in situ rather than using simpler models which do not provide a complete mechanistic insight into the role of phospholipids in membrane dynamics.
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October 2014
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Conference Article|
September 18 2014
Principle of duality in phospholipids: regulators of membrane morphology and dynamics
Banafshé Larijani;
Banafshé Larijani
1
*Cell Biophysics Laboratory, Ikerbasque, Basque Foundation for Science and Unidad de Biofísica (CSIC-UPV/EHU), Barrio de Sarriena s/n, 48940 Leioa, Spain
1Correspondence may be addressed to either of these authors (emailbanafshe.larijani@ikerbasque.org or dlpoccia@amherst.edu).
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Fadi Hamati;
Fadi Hamati
†Department of Biology, Amherst College, Amherst, MA 01002, U.S.A.
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Aupola Kundu;
Aupola Kundu
†Department of Biology, Amherst College, Amherst, MA 01002, U.S.A.
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Gary C. Chung;
Gary C. Chung
*Cell Biophysics Laboratory, Ikerbasque, Basque Foundation for Science and Unidad de Biofísica (CSIC-UPV/EHU), Barrio de Sarriena s/n, 48940 Leioa, Spain
‡Electron Microscopy Unit, Cancer Research UK London Research Institute, 44 Lincoln's Inn Fields, London WC2A 3LY, U.K.
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Marie-Charlotte Domart;
Marie-Charlotte Domart
‡Electron Microscopy Unit, Cancer Research UK London Research Institute, 44 Lincoln's Inn Fields, London WC2A 3LY, U.K.
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Lucy Collinson;
Lucy Collinson
‡Electron Microscopy Unit, Cancer Research UK London Research Institute, 44 Lincoln's Inn Fields, London WC2A 3LY, U.K.
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Dominic L. Poccia
Dominic L. Poccia
1
†Department of Biology, Amherst College, Amherst, MA 01002, U.S.A.
1Correspondence may be addressed to either of these authors (emailbanafshe.larijani@ikerbasque.org or dlpoccia@amherst.edu).
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Publisher: Portland Press Ltd
Received:
August 14 2014
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2014 Biochemical Society
2014
Biochem Soc Trans (2014) 42 (5): 1335–1342.
Article history
Received:
August 14 2014
Citation
Banafshé Larijani, Fadi Hamati, Aupola Kundu, Gary C. Chung, Marie-Charlotte Domart, Lucy Collinson, Dominic L. Poccia; Principle of duality in phospholipids: regulators of membrane morphology and dynamics. Biochem Soc Trans 1 October 2014; 42 (5): 1335–1342. doi: https://doi.org/10.1042/BST20140224
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