Glycolysis is an ancient biochemical pathway that breaks down glucose into pyruvate to produce ATP. The structural and catalytic properties of glycolytic enzymes are well-characterized. However, there is growing appreciation that these enzymes participate in numerous moonlighting functions that are unrelated to glycolysis. Recently, chemical genetics has been used to discover novel moonlighting functions in glycolytic enzymes. In the present mini-review, we introduce chemical genetics and discuss how it can be applied to the discovery of protein moonlighting. Specifically, we describe the application of chemical genetics to uncover moonlighting in two glycolytic enzymes, enolase and glyceraldehyde dehydrogenase. This led to the discovery of moonlighting roles in glucose homoeostasis, cancer progression and diabetes-related complications. Finally, we also provide a brief overview of the latest progress in unravelling the myriad moonlighting roles for these enzymes.
Conference Article| November 17 2014
Chemical genetics and its application to moonlighting in glycolytic enzymes
Darren R. Williams
Darren R. Williams 2
*New Drug Targets Laboratory, School of Life Sciences, Gwangju Institute of Science and Technology, 1 Oryong-Dong, Buk-Gu, Gwangju 500-712, Republic of Korea
2To whom correspondence should be addressed (firstname.lastname@example.org).
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Da-Woon Jung, Woong-Hee Kim, Darren R. Williams; Chemical genetics and its application to moonlighting in glycolytic enzymes. Biochem Soc Trans 1 December 2014; 42 (6): 1756–1761. doi: https://doi.org/10.1042/BST20140201
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