Naturally occurring DNA is encoded by the four nucleobases adenine, cytosine, guanine and thymine. Yet minor chemical modifications to these bases, such as methylation, can significantly alter DNA function, and more drastic changes, such as replacement with unnatural base pairs, could expand its function. In order to realize the full potential of DNA in therapeutic and synthetic biology applications, our ability to ‘write’ long modified DNA in a controlled manner must be improved. This review highlights methods currently used for the synthesis of moderately long chemically modified nucleic acids (up to 1000 bp), their limitations and areas for future expansion.
Review Article| June 09 2016
Synthesis of chemically modified DNA
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Arun Shivalingam, Tom Brown; Synthesis of chemically modified DNA. Biochem Soc Trans 15 June 2016; 44 (3): 709–715. doi: https://doi.org/10.1042/BST20160051
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