The determination of detailed 3D structures of large and transient multicomponent complexes remains challenging. Here I describe the approaches that were used and developed by our laboratory to achieve structure solution of eukaryotic transcription complexes. I hope this collection serves as a resource for structural biologists seeking solutions for difficult structure determination projects.
Review Article| August 15 2016
Structure determination of transient transcription complexes
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Patrick Cramer; Structure determination of transient transcription complexes. Biochem Soc Trans 15 August 2016; 44 (4): 1177–1182. doi: https://doi.org/10.1042/BST20160079
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