Mass spectrometry (MS) has long been used to study proteins mainly via sequence identification and quantitation of expression abundance. In recent years, MS has emerged as a tool for structural biology. Intact protein structural analysis has been enabled by the development of methods such as native MS, top-down proteomics, and ion mobility MS. Other MS-based structural methods include affinity purification MS, chemical cross-linking, and protein footprinting. These methods have enabled the study of protein–protein and protein–ligand interactions and regions of conformational change. The coupling of MS with liquid chromatography has permitted the analysis of complex samples. This bottom-up proteomics workflow enables the study of protein structure in the native cellular environment and provides structural information across the proteome. It has been demonstrated that the crowded environment of the cell affects protein binding interactions and affinities. Performing studies in this complex environment is essential for understanding the functional roles of proteins. MS-based structural methods permit analysis of samples such as cell lysates, intact cells, and tissue to provide a more physiological view of protein structure. This mini-review discusses the various MS-based methods that can be used for proteome-wide structural studies and highlights some of their application.
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June 2020
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SMAD-dependent and SMAD-independent BMP9 signalling pathways during osteogenesis. For more information, see the article by Liu and colleagues in this issue (pp. 1269–1268). The image was provided by Dingming Huang.
Review Article|
May 15 2020
Mass spectrometry-based methods for structural biology on a proteome-wide scale
Lisa M. Jones
Department of Pharmaceutical Sciences, University of Maryland, Baltimore, MD 21201, U.S.A.
Correspondence: Lisa M. Jones ([email protected])
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Publisher: Portland Press Ltd
Received:
February 17 2020
Revision Received:
April 14 2020
Accepted:
April 16 2020
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society
2020
Biochem Soc Trans (2020) 48 (3): 945–954.
Article history
Received:
February 17 2020
Revision Received:
April 14 2020
Accepted:
April 16 2020
Citation
Lisa M. Jones; Mass spectrometry-based methods for structural biology on a proteome-wide scale. Biochem Soc Trans 30 June 2020; 48 (3): 945–954. doi: https://doi.org/10.1042/BST20190794
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