Super-resolution microscopy has revolutionised the way we observe biological systems. These methods are now a staple of fluorescence microscopy. Researchers have used super-resolution methods in myriad systems to extract nanoscale spatial information on multiple interacting parts. These methods are continually being extended and reimagined to further push their resolving power and achieve truly single protein resolution. Here, we explore the most recent advances at the frontier of the ‘super-resolution’ limit and what opportunities remain for further improvements in the near future.
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Review Article| February 18 2021
Pushing the super-resolution limit: recent improvements in microscopy below the diffraction limit
D. J. Nieves;
D. J. Nieves
1Institute of Immunology and Immunotherapy, College of Medical and Dental Sciences, University of Birmingham, Birmingham B15 2TT, U.K.
2Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham and University of Nottingham, Midlands, University of Birmingham, Birmingham B15 2TT, U.K.
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D. J. Nieves, M. A. B. Baker; Pushing the super-resolution limit: recent improvements in microscopy below the diffraction limit. Biochem Soc Trans 26 February 2021; 49 (1): 431–439. doi: https://doi.org/10.1042/BST20200746
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