MVB (multivesicular body) formation occurs when the limiting membrane of an endosome invaginates into the intraluminal space and buds into the lumen, bringing with it a subset of transmembrane cargoes. Exvagination of the endosomal membrane from the cytosol is topologically similar to the budding of retroviral particles and cytokinesis, wherein membranes bud away from the cytoplasm, and the machinery responsible for MVB sorting has been implicated in these phenomena. The AAA (ATPase associated with various cellular activities) Vps4 (vacuolar protein sorting 4) performs a critical function in the MVB sorting pathway. Vps4 appears to dissociate the ESCRTs (endosomal sorting complexes required for transport) from endosomal membranes during the course of MVB sorting, but it is unclear how Vps4 ATPase activity is synchronized with ESCRT release. We have investigated the mechanisms by which ESCRT components stimulate the ATPase activity of Vps4. These studies support a model wherein Vps4 activity is subject to spatial and temporal regulation via distinct mechanisms during MVB sorting.
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February 2009
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Conference Article|
January 20 2009
Regulation of Vps4 ATPase activity by ESCRT-III
Brian A. Davies;
Brian A. Davies
1Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, 200 First Street S.W., Rochester, MN 55905, U.S.A.
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Ishara F. Azmi;
Ishara F. Azmi
1Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, 200 First Street S.W., Rochester, MN 55905, U.S.A.
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David J. Katzmann
David J. Katzmann
1
1Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, 200 First Street S.W., Rochester, MN 55905, U.S.A.
1To whom correspondence should be addressed (email Katzmann.David@mayo.edu).
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Publisher: Portland Press Ltd
Received:
July 18 2008
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© The Authors Journal compilation © 2009 Biochemical Society
2009
Biochem Soc Trans (2009) 37 (1): 143–145.
Article history
Received:
July 18 2008
Citation
Brian A. Davies, Ishara F. Azmi, David J. Katzmann; Regulation of Vps4 ATPase activity by ESCRT-III. Biochem Soc Trans 1 February 2009; 37 (1): 143–145. doi: https://doi.org/10.1042/BST0370143
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