Sorting internalized proteins and lipids back to the cell surface controls the supply of molecules throughout the cell and regulates integral membrane protein activity at the surface. One central process in mammalian cells is the transit of cargo from endosomes back to the plasma membrane (PM) directly, along a route that bypasses retrograde movement to the Golgi. Despite recognition of this pathway for decades we are only beginning to understand the machinery controlling this overall process. The budding yeast Saccharomyces cerevisiae, a stalwart genetic system, has been routinely used to identify fundamental proteins and their modes of action in conserved trafficking pathways. However, the study of cell surface recycling from endosomes in yeast is hampered by difficulties that obscure visualization of the pathway. Here we briefly discuss how recycling is likely a more prevalent process in yeast than is widely appreciated and how tools might be built to better study the pathway.
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April 2016
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Cover Image
Endoplasmic reticulumendosome contact sites. This pseudo-colored electron microscopy image shows the formation of inter-organelle membrane contact sites between late endosomes (magenta) and the endoplasmic reticulum (ER; green). This tethering results from the interaction between two ER-anchored proteins (VAP-A and VAP-B) and the late endosomeanchored protein STARD3NL. Mitochondria: brown; nucleus: blue. For further details see pp. 493-498. Image kindly provided by Fabien Alpy. - PDF Icon PDF LinkTable of Contents
Review Article|
April 11 2016
Cell surface recycling in yeast: mechanisms and machineries
Chris MacDonald;
Chris MacDonald
1
*Molecular Physiology and Biophysics, University of Iowa, Iowa City, IA 52242, U.S.A.
1To whom correspondence should be addressed (email chris-macdonald@uiowa.edu).
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Robert C. Piper
Robert C. Piper
*Molecular Physiology and Biophysics, University of Iowa, Iowa City, IA 52242, U.S.A.
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Publisher: Portland Press Ltd
Received:
January 26 2016
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2016 Authors; published by Portland Press Limited
2016
Biochem Soc Trans (2016) 44 (2): 474–478.
Article history
Received:
January 26 2016
Citation
Chris MacDonald, Robert C. Piper; Cell surface recycling in yeast: mechanisms and machineries. Biochem Soc Trans 15 April 2016; 44 (2): 474–478. doi: https://doi.org/10.1042/BST20150263
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