Akt is an essential protein kinase activated downstream of phosphoinositide 3-kinase and frequently hyperactivated in cancer. Canonically, Akt is activated by phosphoinositide-dependent kinase 1 and mechanistic target of rapamycin complex 2, which phosphorylate it on two regulatory residues in its kinase domain upon targeting of Akt to the plasma membrane by PI(3,4,5)P3. Recent evidence, however, has shown that, in addition to phosphorylation, Akt activity is allosterically coupled to the engagement of PI(3,4,5)P3 or PI(3,4)P2 in cellular membranes. Furthermore, the active membrane-bound conformation of Akt is protected from dephosphorylation, and Akt inactivation by phosphatases is rate-limited by its dissociation. Thus, Akt activity is restricted to membranes containing either PI(3,4,5)P3 or PI(3,4)P2. While PI(3,4,5)P3 has long been associated with signaling at the plasma membrane, PI(3,4)P2 is gaining increasing traction as a signaling lipid and has been implicated in controlling Akt activity throughout the endomembrane system. This has clear implications for the phosphorylation of both freely diffusible substrates and those localized to discrete subcellular compartments.
Akt at a glance—the primary effector of PI3K signaling
Akt is one of the primary effectors of phosphoinositide 3-kinase (PI3K) signaling, regulating myriad processes including growth, proliferation, metabolism, and cell survival. Stimulation of G-protein-coupled receptors (GPCRs) or receptor tyrosine kinases (RTKs) through binding of extracellular growth factors or hormones  on the cell surface promotes downstream activation of class I PI3K (Figure 1). PI3K catalyzes the phosphorylation of phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2) to phosphatidylinositol-3,4,5-trisphosphate [PI(3,4,5)P3] , while phosphatidylinositol-3,4-bisphosphate [PI(3,4)P2] is produced through either dephosphorylation of PI(3,4,5)P3 by the lipid phosphatase SHIP at the plasma membrane [3,4], or by phosphorylation of phosphatidylinositol-4-phosphate [PI(4)P] by class II PI3K [5,6]. By virtue of its pleckstrin homology (PH) domain, which binds both PI(3,4,5)P3 and PI(3,4)P2[7–9], Akt can transduce the PI3K output signal into a downstream response.
Akt at a glance – the primary effector of PI3K signaling.
There are three mammalian isoforms of Akt (Akt1, 2 and 3), which share a conserved domain arrangement of an N-terminal PH domain followed by a C-terminal serine/threonine kinase domain (Figure 2A). While not the focus of this article, the physiological and pathological roles of Akt isoforms have been reviewed in  and an emerging body of evidence also links signaling specificity to isoform-specific subcellular localization or isoform-specific substrates .
Switching Akt on and off.
Dysregulation of PI3K/Akt signaling is associated with various pathologies including cancer, overgrowth disorders, and metabolic disease . Oncogenic mutations in PIK3CA, the gene encoding the catalytic subunit of PI3K (p110α), are second only to Ras in frequency, both of which drive the overproduction of PI(3,4,5)P3 at the plasma membrane and result in an amplification of Akt signaling [12,13]. Mutations in the tumor suppressor phosphatase and tensin homolog (PTEN) are the third most common in human cancers . Loss of PTEN, which terminates PI3K signaling by dephosphorylating the 3′-phosphoinositide products of PI3K, leads to an increase in PI(3,4,5)P3 at the membrane and hyperactivated Akt [15,16]. These gain- and loss-of-function mutations are also found in several overgrowth disorders [17,18], therefore highlighting the requirement for lipid signaling to be tightly regulated.
This article reviews our current knowledge about the fundamental mechanisms that control Akt activity in the cell. We focus primarily on recent advances in our understanding of how the lipid second messengers PI(3,4,5)P3 and PI(3,4)P2 regulate Akt activity and how these lipids are generated, distributed, and turned over in cells. In addition to its regulation by lipids, we discuss the activation of Akt by phosphorylation and its inactivation by dephosphorylation, and the mechanisms by which Akt is pathologically hyperactivated. Finally, we provide a perspective on the open questions that require further investigation.
Switching Akt on
Akt is a serine/threonine kinase of the AGC family, the hallmark of which is the presence of a C-terminal regulatory tail containing one or more phosphorylation sites . In the absence of a lipid stimulus, Akt is constitutively phosphorylated on T450, in its so-called turn motif, which is one of two phosphorylation sites in its C-terminal tail (Figure 2A). Turn motif phosphorylation stabilizes Akt and protects it from degradation by promoting the interaction of the tail with a conserved basic patch on the surface of the N-lobe of the kinase domain  (Figure 2B, left panel).
The generation of either PI(3,4,5)P3 or PI(3,4)P2 initiates translocation of Akt to the plasma membrane, leading to its phosphorylation on two residues in its activation loop (T308 in Akt1) and hydrophobic motif (S473 in Akt1), by phosphoinositide-dependent kinase 1 (PDK1) [21,22] and mechanistic target of rapamycin complex 2 (mTORC2) , respectively (Figure 2B, middle panel). PDK1 is co-recruited to the plasma membrane by virtue of its own PH domain, which, like Akt, binds to both PI(3,4,5)P3 and PI(3,4)P2 and drives PDK1 activation by homodimerization and trans-autophosphorylation [25,26]. mTORC2, on the other hand, is recruited to the plasma membrane by the PH domain of its obligate component Sin1 in a PI(3,4,5)P3-independent manner, leading to the suggestion that Akt hydrophobic motif phosphorylation is driven by the recruitment of Akt to constitutively active mTORC2 localized on distinct subcellular compartments .
Phosphorylation of the hydrophobic motif of the AGC kinases S6 kinase (S6K) and serum and glucocorticoid-regulated kinase 1 (SGK1), or a phosphomimetic at this site in atypical protein kinase C (aPKC) and protein kinase C-related kinase 2 (PRK2), enhances their activation by PDK1 [28,29], suggesting that hydrophobic motif phosphorylation by mTORC2 precedes activation loop phosphorylation by PDK1. While PDK1 itself lacks a hydrophobic motif, a hydrophobic pocket in the kinase domain of PDK1, called the PIF (PDK1-interacting fragment) pocket, binds to peptides containing a hydrophobic motif . Phosphorylation of a conserved serine residue in the hydrophobic motif consensus sequence FXXFS or the presence of a phosphomimetic in place of the serine promotes this interaction [28,29], leading to the concept that hydrophobic motif phosphorylation of PDK1 substrates creates a docking site for PDK1 recruitment, thereby driving activation loop phosphorylation. While Akt was initially suggested to be activated by PDK1 independently of hydrophobic motif phosphorylation, the kinase domain of Akt bearing a phosphomimetic aspartate in its hydrophobic motif is a significantly better substrate for PDK1, and this is abrogated by mutation of the PIF pocket in PDK1 . More recent work indicates that Akt bearing a phosphomimetic aspartate in its hydrophobic motif is also more efficiently phosphorylated by PDK1 in solution [31,32] and that disruption of the PIF pocket mechanism in Akt impacts Akt activation and sensitizes Akt to PDK1 inhibitors . On balance, all indications are that activation loop phosphorylation of Akt by PDK1 depends on both its co-recruitment to PI(3,4,5)P3- or PI(3,4)P2-containing membranes and phosphorylation of its hydrophobic motif by mTORC2. Hydrophobic motif phosphorylation likely drives PDK1-mediated activation loop phosphorylation by both strengthening the interaction of PDK1  with Akt and possibly positioning Akt optimally for catalysis.
In addition to enhancing activation loop phosphorylation by PDK1, phosphorylation of the hydrophobic motif promotes docking to a hydrophobic pocket in the kinase domain of Akt itself, thereby driving a disorder-to-order transition of the hydrophobic motif and the αC helix. This leads to the formation of a network of stabilizing interactions between the αC helix, ATP, and residues in the activation segment including pT308, which serve to immobilize the activation loop on the surface of the kinase domain [34,35]. However, precisely what triggers the dissociation of the phosphorylated hydrophobic motif of Akt from PDK1 and its subsequent intramolecular engagement is currently unknown. Activation loop phosphorylation by PDK1 may be a prerequisite for pS473 binding to the PIF pocket of Akt, in which case the intramolecular interaction may be favored post-activation loop phosphorylation, though this will need careful investigation. Nevertheless, dual phosphorylation of Akt effectively creates the substrate-binding cleft and organizes the catalytic residues essential for phospho-transfer  (Figure 2B, right panel; Figure 2C, left panel). A recent study suggests that S473 phosphorylation is stabilized by a basic patch in the PH–kinase domain linker containing R144 (Figure 2B, right panel, orange inset box), mutation of which results in a ∼50-fold reduction in catalytic efficiency . Since the side chain of R144 cannot reach the side chain of an aspartate phosphomimetic, these observations raise the obvious question of whether D473 is a faithful mimic of pS473, despite its use in multiple cellular and in vitro studies.
That phosphorylation of Akt was required for its activity was recognized from the very beginning. However, early studies provided contradictory data on whether PI(3,4,5)P3 binding also contributed to an increase in Akt activity by relieving an autoinhibitory interaction between its PH and kinase domains. Some studies have hinted at such a conformation: Akt binding to PI(3,4,5)P3 enhances activation loop phosphorylation by PDK1  and deletion of the PH domain of Akt promotes hydrophobic motif phosphorylation in an mTORC2-independent manner in Sin1 knockout fibroblasts . Other studies including FRET and computational modeling [37–39], as well as the crystal structure of a C-terminally truncated Akt1 in complex with an allosteric inhibitor  and a follow-up mutational study  have strongly suggested the existence of an inactive conformation. However, it has only recently been demonstrated that Akt is directly activated in vitro by both PI(3,4,5)P3 and PI(3,4)P2 [42,43]. Mechanistically, lipid binding displaces the PH domain from the catalytic cleft, leading to an 8-fold increase in substrate binding. Mutation of two evolutionarily invariant, surface-exposed residues on the surface of the kinase domain, D323 and D325, uncouple kinase activity from PI(3,4,5)P3 and lead to Akt hyperphosphorylation, which is accompanied by a further 5-fold increase in affinity for the substrate. Displacement of the PH domain from the kinase domain results in enhanced membrane binding both in vitro and in vivo, indicating that the autoinhibitory interface also sequesters the PI(3,4,5)P3-binding pocket in an inaccessible conformation. Together, binding to PI(3,4,5)P3 and phosphorylation lead to a combined 40-fold increase in substrate binding. Consistent with these observations, Akt was only observed to form a complex with a model substrate in vivo when bound to PI(3,4,5)P3- or PI(3,4)P2-containing membranes .
Recent small-angle X-ray scattering (SAXS) and hydrogen–deuterium exchange mass spectrometry (HDX-MS) experiments have elucidated the conformational changes accompanying PI(3,4,5)P3 binding . In the absence of PI(3,4,5)P3, phosphorylation of Akt is impeded, since both the activation loop and the hydrophobic motif are sequestered in the autoinhibited conformation. Binding of Akt to either PI(3,4,5)P3 or PI(3,4)P2 relieves autoinhibition by the PH domain by displacing it from the catalytic cleft and concomitantly liberating both the activation loop and hydrophobic motif for phosphorylation (Figure 2B, middle panel). Stoichiometric activation loop phosphorylation, at least in the context of a hydrophobic motif phosphomimetic (D473), is insufficient to overcome the dependency on lipid binding for full activation. In summary, Akt functions like a logic gate: both PI(3,4,5)P3/PI(3,4)P2 binding and phosphorylation are required to activate the kinase.
Docking of the phosphorylated activation loop and hydrophobic motif to the kinase domain has implications for the processivity of Akt signaling and its inactivation by phosphatases. Several studies have demonstrated that binding of ATP, but not ADP, protects Akt from dephosphorylation through caging of the phosphorylated activation loop and hydrophobic motif, which results in their restricted accessibility [44–46]. In other words, the same network of interactions that stabilize the active conformation of Akt protects it from dephosphorylation. Binding of ATP-competitive, but not allosteric inhibitors, also locks Akt in a hyperphosphorylated state and protects it from dephosphorylation [44,47]. Structural and mutational analyses in combination with molecular dynamics simulations have shown that the phosphorylation of T308 leads to formation of stabilizing contacts with R273 and H194, two residues in the nucleotide-binding pocket, which shield the regulatory residue from dephosphorylation [44,46]; mutation of either residue to alanine renders T308 susceptible to phosphatases . Importantly, mutation of Q218 in the kinase domain, which forms a hydrogen bond with the phosphate group of pS473 (Figure 2B, right panel) also leads to decreased steady-state Akt phosphorylation . In conclusion, phosphorylation of Akt results in a set of intramolecular interactions in which pT308 and pS473 co-operate to form the substrate-binding cleft and organize the catalytic machinery; as a consequence, they are protected from dephosphorylation in the presence of ATP. Since ATP concentrations in the cell are typically an order of magnitude greater than ADP, it seems likely that the ADP produced in a single catalytic cycle would be rapidly replaced with ATP. In this scenario, Akt would be permissive for iterative cycles of substrate phosphorylation, providing it remains in its active, membrane-bound conformation (Figure 2B, right panel; Figure 2C, left panel).
The ATP-dependent protection of phosphorylated Akt from dephosphorylation raises the obvious question of whether Akt requires PI(3,4,5)P3 or PI(3,4)P2 for activity once phosphorylated. While we have shown in two separate studies that phosphorylated Akt depends on PI(3,4,5)P3 or PI(3,4)P2 for full activity [42,43], a more recent study failed to observe the activation of Akt by PI(3,4,5)P3 . Instead, the authors propose that phosphorylation of S473 in the hydrophobic motif promotes the disengagement of the PH domain from the kinase domain by facilitating an interaction with R144 in the PH–kinase linker. In this way, Akt could be activated by phosphorylation in the absence of PI(3,4,5)P3. However, there are significant problems with this model. First of all, the model relies on phosphorylation of the hydrophobic motif prior to PH domain disengagement when evidence suggests that these two events occur in the opposite order: deletion of the PH domain of Akt promotes hydrophobic motif phosphorylation in an mTORC2-independent manner , while PI(3,4,5)P3 binding results in displacement of the unphosphorylated hydrophobic motif from the kinase domain , suggesting that membrane binding elicits a conformational change that exposes the tail for phosphorylation. Secondly, the model implies that a single additional hydrogen bond made by pS473 to R144 is sufficient to disrupt an interface of ∼1500 Å2 buried surface area , which seems energetically unlikely.
So why do the authors of this study fail to observe Akt activation by PI(3,4,5)P3 or PI(3,4)P2? We previously demonstrated that the high concentration of magnesium (2–10 mM) routinely used in kinase assays inhibits Akt1 binding to PI(3,4,5)P3-containing liposomes . When adjusted to levels that support ∼70% binding (0.2 mM), Akt is activated in a concentration-dependent manner by both PI(3,4,5)P3 and PI(3,4)P2, where the activity curve precisely mirrors the binding curve. The authors of the latest study dispute the influence of magnesium on PI(3,4,5)P3 binding , but their conclusion is based on analysis of the PH domain binding to soluble PI(3,4,5)P3 and not on the binding of full-length Akt1 to PI(3,4,5)P3-containing liposomes, which is more representative of physiological conditions. Furthermore, to activate Akt, the authors use a simple DOPC-PI(3,4,5)P3 liposome mixture for which they do not show evidence of Akt binding. At least in our hands, such a lipid composition does not support robust binding of full-length Akt1, which may explain the authors’ failure to observe activation by PI(3,4,5)P3.
In summary, the requirement for PI(3,4,5)P3 or PI(3,4)P2 for Akt activity is supported in vitro by biochemistry [42,43], mutagenesis [41–43], solution mapping of the autoinhibitory conformation , and a crystal structure in complex with an allosteric inhibitor . In vivo, it is supported by diffusion measurements of the enzyme–substrate complex . Nevertheless, the use of a phosphomimetic in the hydrophobic motif (D473)  is clearly only a proxy for S473 phosphorylation and further work will undoubtedly be required to unambiguously resolve the question of whether hydrophobic motif phosphorylation can activate Akt in a cellular context independently of either of these two lipids.
Switching Akt off
The canonical pathway to Akt inactivation centers on the turnover of PI(3,4,5)P3 and PI(3,4)P2 by the lipid phosphatases PTEN and inositol polyphosphate 4-phosphatase (INPP4), respectively (Figure 2C, middle panel). PTEN hydrolyzes the 3′-phosphate of PI(3,4,5)P3 at the plasma membrane (reviewed in [15,16]), while INPP4 catalyzes the dephosphorylation of PI(3,4)P2 to PI(3)P at the plasma membrane and on endomembranes [48,49]. Turnover of PI(3,4,5)P3 and PI(3,4)P2 limits the activation of both Akt and PDK1 by phosphorylation. Evidence also indicates that it limits Akt activity by returning Akt to its autoinhibited conformation in which its substrate-binding cleft is blocked by its PH domain  (Figure 2C, middle panel).
We recently demonstrated that stoichiometric activation loop phosphorylation in combination with a hydrophobic motif phosphomimetic (D473) is insufficient to override the dependency on PI(3,4,5)P3 or PI(3,4)P2 for full activity . HDX-MS analysis of this species in the absence of PI(3,4,5)P3 indicates that the autoinhibitory interface between the PH and kinase domains is maintained, but that the phosphorylated activation loop is considerably more labile than its unphosphorylated counterpart . This prompted us to hypothesize that dissociation from the membrane triggers PH domain-mediated autoinhibition, which expels the activation loop from its docked conformation on the kinase domain (Figure 3, middle panel). To test whether dissociation could prime Akt for dephosphorylation, we examined the dephosphorylation kinetics of full-length Akt and its isolated kinase domain. Significantly, only the kinase domain is protected from dephosphorylation in the presence of ATP . We concluded that the PH domain promotes Akt dephosphorylation. This provides a potential explanation for our earlier observations that Akt dephosphorylation in cells is rate-limited by its dissociation from the membrane . Together, these findings suggest that Akt inactivation may actually occur in the cytosol, post-membrane dissociation.
Spatiotemporal dynamics of PI(3,4,5)P3 and PI(3,4)P2.
Two phosphatases have been implicated in the dephosphorylation of Akt: protein phosphatase 2A (PP2A) and the PH domain leucine-rich repeat protein phosphatase (PHLPP) [51,52] (Figure 2C, right panel). The specificity of PP2A for its substrates is generally accomplished by incorporation of specific regulatory subunits into a heterotrimeric holoenzyme assembly . PP2A specifically dephosphorylates Akt at T308 through incorporation of the regulatory subunit B55α , but we still lack a mechanistic understanding of T308-specific dephosphorylation. PHLPP has been proposed to selectively dephosphorylate S473 in the hydrophobic motif of Akt . However, it is unclear how the specificity for S473 over T308 is achieved or why hydrophobic motif dephosphorylation in vivo would not affect T308 phosphorylation given their synergistic stabilization and similar dephosphorylation kinetics . Furthermore, PHLPP is a membrane-associated phosphatase, either by directly binding to lipids via its PH domain  or indirectly through association of its PDZ ligand with membrane-resident scaffolding proteins [56,57]. Logically, this implies that substrate dephosphorylation happens at, or in close proximity to, the membrane. However, since Akt is protected from dephosphorylation when bound to PI(3,4,5)P3 and ATP , it raises the question of whether Akt is a substrate for dephosphorylation when membrane bound. The location and conditions under which Akt is dephosphorylated should, therefore, be an important focus of future studies.
Spatiotemporal dynamics of PI(3,4,5)P3 and PI(3,4)P2
Accumulating evidence indicates that the key to both Akt activation and activity is PI(3,4,5)P3 or PI(3,4)P2. It therefore follows that subcellular Akt activity will be governed by the spatial and temporal distribution of these two lipids. In this section, we discuss where, when, and how these lipids are produced at different subcellular compartments, the enzymes that are responsible for both their production and turnover, and the implications for the activation of Akt and its substrate specificity.
In serum-starved cells, PI(3,4,5)P3 accounts for <0.1 mol% of all phospholipids, while growth factor stimulation transiently increases PI(3,4,5)P3 levels to ∼0.5 mol% . Following growth factor binding, RTKs are rapidly internalized and degraded via the lysosomal pathway or recycled to the plasma membrane . However, there is mounting evidence that signaling of the internalized receptors is actually sustained at endomembrane sites. PI(3,4)P2 itself has been found in endocytic vesicles [5,59], early endosomes [4,59], recycling endosomes , and late endosomes/lysosomes  (Figure 3), highlighting the existence of potential Akt signaling hubs in the cell interior. With respect to Akt activation, both PDK1 and mTORC2 have been found on endosomes [27,62]. Appl1, a protein involved in vesicle trafficking, Akt2, and its substrate GSK3β co-localize on endosomal membranes in a PI(3,4)P2-dependent manner , while another study indicates that class II PI3K-C2γ is responsible for sustained and isoform-specific Akt2 signaling on Rab5-positive early endosomes in glucose homeostasis . Ratiometric imaging of PI(3,4,5)P3 and PI(3,4)P2 using high specificity fluorescence sensors has revealed that these lipids exhibit distinct spatiotemporal distributions in response to growth factor stimulation and that this leads to the differential regulation of Akt isoforms .
Previously believed to be just a byproduct of PI(3,4,5)P3 dephosphorylation, PI(3,4)P2 is increasingly being recognized as a signaling molecule in its own right, in addition to its known roles in membrane trafficking [5,59,65]. Indeed, Akt is activated in vitro to the same extent by PI(3,4,5)P3 and PI(3,4)P2 , which is consistent with the crystal structure of the PH domain of Akt1 in complex with inositol-1,3,4,5-tetrakisphosphate (IP4), in which the 5′-phosphate is not specifically recognized . Soluble IP4, however, does not activate Akt in vitro . These findings emphasize the importance of both the 3′-phosphate and the membrane environment as essential for Akt activity. Canonically, PI(3,4)P2 is generated by class II PI3K through 3′-phosphorylation of PI(4)P . However, two recent studies have shown that the majority of endomembrane PI(3,4)P2 is actually produced through SHIP-mediated dephosphorylation of plasma membrane PI(3,4,5)P3 rather than by phosphorylation of PI(4)P [4,66]. Consistent with actively signaling pools of PI(3,4)P2 in the cell interior, targeting of PTEN to endomembranes has also been shown to convert PI(3,4)P2 to PI(4)P  in addition to its canonical role as a PI(3,4,5)P3 phosphatase.
Akt has been reported to phosphorylate more than 100 substrates, of which only a small fraction have been validated in vitro and in vivo . Several of these substrates, including RAF1 and eNOS, localize to the plasma membrane. Other substrates, such as GSK3β localize to endosomes , while TSC2 and PRAS40, which is an inhibitory component of the mTORC1 complex [68,69], localize to the lysosome . WNK and AS160 are found on both the plasma membrane and secretory vesicles [71,72] (Figure 3). While several substrates of Akt are found in the nucleus, which has led many to believe that Akt is active in the nucleus, evidence for nuclear PI(3,4,5)P3 or PI(3,4)P2 remains scarce . Of these substrates, FOXO, Chk1, p27, MDM2, and IKKα shuttle between the nucleus and cytoplasm, and in principle could be phosphorylated in the cytoplasm [74,75]. In summary, the phosphorylation of a freely diffusible substrate is entirely consistent with membrane-restricted Akt activity, while the phosphorylation of a membrane-resident substrate will be dependent on the spatial and temporal distribution of PI(3,4,5)P3 or PI(3,4)P2. The local activation of Akt at subcellular compartments other than the plasma membrane will, of course, depend not only on the presence of PI(3,4,5)P3 or PI(3,4)P2 but also on the co-localization of PDK1 and mTORC2. While PDK1 binds to both PI(3,4,5)P3 and PI(3,4)P2 and therefore co-localizes with Akt, the mechanisms governing mTORC2 localization on endomembranes are less clear.
Pathological hyperactivation and inactivation of Akt
The PI3K/Akt signaling pathway can be dysregulated at several different levels, and hot spot mutations in PI3K that up-regulate PI(3,4,5)P3 production or loss/inactivation of PTEN have already been extensively reviewed [2,12,16,76,77]. Importantly, however, several mutations in Akt itself have also been observed, which either disrupt the autoinhibitory interface between the PH and kinase domain and uncouple the enzyme from the lipid stimulus, lead to increased association of Akt with membranes, or inactivate Akt.
Akt was originally identified as a viral oncogene in which a sequence from the gag polyprotein was appended to the N-terminus of Akt . Myristoylation of Akt leads to constitutive membrane association and Akt hyperactivation , presumably by increasing the on-rate of PI(3,4,5)P3 binding.
Mutation of a conserved glutamate in the PH domain (E17K) has been identified in breast cancer  as well as overgrowth disorders such as Proteus syndrome  and hemimegalencephaly . E17K dramatically enhances the affinity of Akt for PI(4,5)P2 , thereby increasing association with the membrane in the absence of PI(3,4,5)P3 . Although it was previously observed that this mutation also weakens the interaction between PH and kinase domains , it was more recently shown that its activity in vitro is still dependent on PI(3,4,5)P3 . It remains to be determined whether E17K, in the context of full-length Akt, associates with the membrane at lower PI(3,4,5)P3 levels due to weakening of the autoinhibitory interface, but this seems a likely explanation for its hyperactivation and oncogenic potential.
From the dependency on PI(3,4,5)P3/PI(3,4)P2 for Akt activity, it logically follows that mutations in the autoinhibitory interface might uncouple Akt activity from the membrane. A screen of mutants in the PH–kinase interface, which was identified in the crystal structure of Akt1 in complex with an allosteric inhibitor , indicated many mutants that exhibited reduced PH–kinase domain association, resistance to allosteric inhibitors, and transforming activity in vivo . Mutation of D323 and D325, surface-exposed and invariant residues on the kinase domain, leads to PI(3,4,5)P3-independent activity in vitro, hyperphosphorylation, and formation of a kinase–substrate complex in the cytosol of cells . Biophysical and biochemical analysis has revealed an extended conformation, displacement of the PH domain from the catalytic cleft, and resistance to dephosphorylation in the presence of ATP . Mutation of D323 in Akt1 has been observed in at least one case of urinary carcinoma  and the equivalent residue D322 in Akt3, the predominant isoform of Akt in the brain, in rare cases of extreme megalencephaly . It should be noted that the pathological manifestation of disease depends on the nature of the mutation in Akt. Those that uncouple Akt activity from PI(3,4,5)P3 will undoubtedly have more severe consequences than those that simply enhance Akt activation by PI(3,4,5)P3, which may explain why they are comparatively rare in cancer .
While the majority of mutations lead to hyperactivation of Akt and increased signaling output, mutation of R274 to histidine in Akt2 inactivates the enzyme, leading to insulin resistance and diabetes . Residing in the catalytic cleft of Akt, R274 is important for co-ordinating the phosphorylated T308 in the activation loop (Figure 2B, right panel). The shorter side chain of histidine cannot make the same stabilizing hydrogen bond, leading to Akt dephosphorylation and inactivation even in the presence of ATP .
Importance of the field: Akt is a critical regulator of a diverse array of processes in the cell that co-ordinate growth, proliferation, and metabolism. Akt activity is up-regulated in a majority of human cancers that arise from aberrant PI3K signaling. Understanding the regulation of Akt by the products of PI3K is, therefore, of paramount importance in our efforts to rationalize both physiological and pathological signal transduction via this pathway.
Summary of current thinking: The dependency of Akt on PI(3,4,5)P3 or PI(3,4)P2 for its activation has long been recognized, but whether it depends on either of these lipids for activity is still somewhat controversial. However, recent studies have clearly demonstrated that lipid binding relieves occlusion of the substrate-binding cleft by the PH domain and that activation loop phosphorylation is insufficient to overcome this requirement. Diffusion measurements of Akt inside cells have revealed the presence of a substantial pool of endomembrane-associated Akt consistent with recent studies demonstrating the presence of PI(3,4)P2 in endosomal compartments. Consistent with a requirement for PI(3,4,5)P3 or PI(3,4)P2, active Akt in the cell interior appears to be almost exclusively associated with a membrane compartment. There is currently little evidence for exclusively nuclear Akt substrates, nuclear pools of membrane-embedded PI(3,4,5)P3 or PI(3,4)P2, or active Akt in the nucleus.
Future directions: With the exception of mTORC2 localization, Akt activation by PI(3,4,5)P3 or PI(3,4)P2 is now well understood. Future studies will help refine our understanding of how PI3K signaling is co-ordinated with mTORC2 phosphorylation of Akt; this is particularly important since hydrophobic motif phosphorylation appears to be a prerequisite for PDK1-mediated activation loop phosphorylation and is required for ATP-dependent stabilization of the active conformation on the membrane. Further studies are also required to unambiguously determine whether hydrophobic motif phosphorylation can activate Akt independently of lipids. The results of these studies will not only inform our understanding of Akt signaling but also of other AGC kinases, such as PKC and S6K, that depend on hydrophobic motif phosphorylation. Though the evidence strongly indicates that Akt activity is intrinsically coupled to engagement of PI(3,4,5)P3 or PI(3,4)P2 in membranes, the inactivation of Akt by phosphatases is comparatively poorly understood. We still have much to learn about precisely where, when, and how Akt is switched off.
hydrogen–deuterium exchange mass spectrometry
inositol polyphosphate 4-phosphatase
mechanistic target of rapamycin complex 2
phosphoinositide-dependent kinase 1
PH domain leucine-rich repeat protein phosphatase
protein kinase C
protein phosphatase 2A
phosphatase and tensin homolog
receptor tyrosine kinase
This work was funded by Austrian Science Fund (FWF) grants P28135 and P30584.
The Authors declare that there are no competing interests associated with the manuscript.