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Keywords: CRISPR-Cas
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Biochem Soc Trans (2019) 47 (5): 1499–1510.
Published: 11 October 2019
... bound to a full-length target DNA, the target DNA is not positioned in the catalytic site in any of these structures. This suggests that interactions between the catalytic site and target DNA are transient. The catalytic pocket of CRISPRCas type V-B effector protein Cas12b is highly similar...
Biochem Soc Trans (2016) 44 (5): 1355–1365.
Published: 19 October 2016
... palindromic repeats (CRISPR-Cas) mechanisms will be described, which can be delivered by lentiviral vectors. These mechanisms attack different forms of the viral genome, the RNA and DNA, respectively, but both mechanisms act in a strictly sequence-specific manner. Early RNAi experiments demonstrated profound...