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Keywords: Escherichia coli
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Articles
Biochem Soc Trans (2019) 47 (3): 839–845.
Published: 12 June 2019
.... Here, we review recent data indicating that the activity of the Escherichia coli lac operon promoter also depends upon its chromosomal location. Factors that affect promoter activity include the binding of nucleoid-associated proteins to neighbouring sequences, supercoiling and the activity of...
Articles
Biochem Soc Trans (2019) 47 (2): 755–763.
Published: 10 April 2019
...Douglas F. Browning; Rita E. Godfrey; Kirsty L. Richards; Colin Robinson; Stephen J.W. Busby The Escherichia coli lac operon promoter is widely used as a tool to control recombinant protein production in bacteria. Here, we give a brief review of how it functions, how it is regulated, and how, based...
Articles
Biochem Soc Trans (2018) 46 (6): 1721–1728.
Published: 04 December 2018
... nitrogen starvation has been well studied. The adaptive response to N starvation in Escherichia coli is primarily a ‘scavenging response’, which results in the transcription of genes required for the transport and catabolism of nitrogenous compounds. However, recent genome-scale studies have begun to...
Articles
Biochem Soc Trans (2014) 42 (2): 479–484.
Published: 20 March 2014
... clues as to how the molecular structures of these proteins control metal-activation profiles. 1 email jhonek@uwaterloo.ca 6 1 2014 © The Authors Journal compilation © 2014 Biochemical Society 2014 Clostridium acetobutylicum Escherichia coli glyoxalase metal...
Includes: Multimedia, Supplementary data
Articles
Biochem Soc Trans (2013) 41 (6): 1412–1415.
Published: 20 November 2013
... adaptation process, and focus primarily on the subtype I-E CRISPR–Cas system of Escherichia coli . 1 To whom correspondence may be addressed (email ehudq@post.tau.ac.il ). 24 6 2013 © The Authors Journal compilation © 2013 Biochemical Society 2013 clustered regularly interspaced...
Articles
Biochem Soc Trans (2013) 41 (5): 1288–1293.
Published: 23 September 2013
...Gabriele Deckers-Hebestreit The ATP synthase (F o F 1 ) of Escherichia coli couples the translocation of protons across the cytoplasmic membrane by F o to ATP synthesis or hydrolysis in F 1 . Whereas good knowledge of the nanostructure and the rotary mechanism of the ATP synthase is at hand, the...
Articles
Biochem Soc Trans (2013) 41 (2): 571–575.
Published: 21 March 2013
... or strands through the break. Although all type IA topoisomerases are related at the sequence, structure and mechanism levels, different type IA enzymes do not participate in the same cellular processes. We have studied the mechanism of DNA relaxation by Escherichia coli topoisomerases I and III...
Articles
Biochem Soc Trans (2013) 41 (2): 595–600.
Published: 21 March 2013
...Ian Grainge FtsK is a multifunctional protein, which, in Escherichia coli , co-ordinates the essential functions of cell division, DNA unlinking and chromosome segregation. Its C-terminus is a DNA translocase, the fastest yet characterized, which acts as a septum-localized DNA pump. FtsK's C...
Articles
Biochem Soc Trans (2012) 40 (6): 1222–1226.
Published: 21 November 2012
... methods from the toolbox for chromosome-based heterologous expression in Escherichia coli . 1 To whom correspondence should be addressed (email johannes.gescher@kit.edu ). 8 6 2012 © 2012 The Authors Journal 2012 counter-selection Escherichia coli heterologous multiprotein...
Articles
Biochem Soc Trans (2012) 40 (6): 1475–1479.
Published: 21 November 2012
... of the protein machines within it. Our work focuses on the import mechanism of the group A endonuclease (DNase) colicin ColE9 into Escherichia coli , where we combine in vivo observations with structural, biochemical and biophysical approaches to dissect the molecular mechanism of colicin entry...
Articles
Biochem Soc Trans (2011) 39 (6): 1876–1879.
Published: 21 November 2011
..., we provide a brief introduction into the NO detoxification mechanisms of two members of the Enterobacteriaceae family: Escherichia coli and Salmonella enterica serovar Typhimurium. These are discussed as comparative non-pathogenic and pathogenic model systems in order to investigate the importance of...
Articles
Biochem Soc Trans (2011) 39 (1): 213–215.
Published: 19 January 2011
... oxide) to harmless products. The highest rates of NO reduction by Escherichia coli K-12 were detected after anaerobic growth in the presence of nitrate. Four proteins have been implicated as catalysts of NO reduction: the cytoplasmic sirohaem-containing nitrite reductase, NirB; the periplasmic...
Articles
Biochem Soc Trans (2010) 38 (2): 395–398.
Published: 22 March 2010
...David J. Sherratt; Lidia K. Arciszewska; Estelle Crozat; James E. Graham; Ian Grainge Escherichia coli FtsK is a septum-located DNA translocase that co-ordinates the late stages of cytokinesis and chromosome segregation. Relatives of FtsK are present in most bacteria; in Bacillus subtilis , the...
Articles
Biochem Soc Trans (2008) 36 (6): 1144–1148.
Published: 19 November 2008
... respiration. In Escherichia coli , the fumarate and nitrate reduction transcriptional regulator (FNR) controls this switch. Under O 2 -limiting conditions, FNR binds a [4Fe–4S] 2+ cluster, generating a transcriptionally active dimeric form. Exposure to O 2 results in conversion of the cluster into a [2Fe–2S...
Articles
Biochem Soc Trans (2008) 36 (6): 1409–1413.
Published: 19 November 2008
...Daniel A. Bonsor; Nicola A. Meenan; Colin Kleanthous The translocation of protein toxins into a cell relies on a myriad of protein–protein interactions. One such group of toxins are enzymatic E colicins, protein antibiotics produced by Escherichia coli in times of stress. These proteins subvert...
Articles
Biochem Soc Trans (2008) 36 (5): 1032–1036.
Published: 19 September 2008
...-understood process at the biochemical level, relatively little is known about its operation at the level of the whole-organelle/cell. We observed that a fluorescently labelled terminal oxidase, the cytochrome bd complex, is heterogeneously distributed in the Escherichia coli plasma membrane. This observation...
Articles
Biochem Soc Trans (2008) 36 (5): 971–975.
Published: 19 September 2008
... translocation. Owing to this, a conformation-linked mechanism for redox-driven proton translocation is discussed. By means of electron microscopy, we show that both arms of the Escherichia coli complex I are widened after the addition of NADH but not of NADPH. NADH-induced conformational changes were also...
Articles
Biochem Soc Trans (2008) 36 (5): 1005–1010.
Published: 19 September 2008
...Thomas A. Clarke; Tracey Holley; Robert S. Hartshorne; Jim K. Fredrickson; John M. Zachara; Liang Shi; David J. Richardson The periplasmic nitrite reductase system from Escherichia coli and the extracellular Fe(III) reductase system from Shewanella oneidensis contain multihaem c -type cytochromes...
Articles
Biochem Soc Trans (2008) 36 (4): 754–757.
Published: 22 July 2008
...David C. Grainger; Stephen J.W. Busby A major goal in the study of gene regulation is to untangle the transcription-regulatory networks of Escherichia coli and other ‘simple’ organisms. To do this we must catalogue the binding sites of all transcription factors. ChIP (chromatin immunoprecipitation...
Articles
Biochem Soc Trans (2006) 34 (6): 1231–1235.
Published: 25 October 2006
... cytosolic forms in Escherichia coli . However, selective permeability of E. coli to many substrates and products can cause problems with product recovery when using whole-cell systems. To overcome these problems, we have been developing approaches to facilitate export of functional recombinant haemoproteins...
Articles
Biochem Soc Trans (2006) 34 (6): 1183–1185.
Published: 25 October 2006
... questions, we have expressed and purified five S. coelicolor P450s, four FdRs and six Fdxs in Escherichia coli . Of the purified P450s, CYP105D5 has fatty acid hydroxylation activity in a system reconstituted with putidaredoxin reductase and Fdx4 or with spinach FdR and spinach Fdx, although the...
Articles
Biochem Soc Trans (2006) 34 (1): 91–93.
Published: 20 January 2006
...@bioch.ox.ac.uk ). 14 9 2005 © 2006 The Biochemical Society 2006 c -type cytochrome cytochrome c maturation (Ccm) Escherichia coli haem post-translational modification c -Type cytochromes are characterized by covalent attachment of haem to protein through thioether bonds between...
Articles
Biochem Soc Trans (2006) 34 (1): 133–135.
Published: 20 January 2006
...J.D. Gwyer; D.J. Richardson; J.N. Butt Escherichia coli cytochrome c nitrite reductase is one of a large family of homologous enzymes that are particularly prevalent in pathogenic enterobacteria. The enzymes are periplasmic and in vivo may find themselves challenged by molecules that could enhance...
Articles
Biochem Soc Trans (2006) 34 (1): 200–202.
Published: 20 January 2006
...S. Spiro Exposure of Escherichia coli to nitric oxide (NO) or nitrosating agents causes significant changes in patterns of gene expression. Three recent studies have used microarrays to analyse the response of the E. coli transcriptome to NO and nitrosative stress. Drawing on the array data, I...
Articles
Biochem Soc Trans (2005) 33 (4): 836–839.
Published: 01 August 2005
... exclusively works as a proton pump. Recently, it has been proposed that complex I from Klebsiella pneumoniae and Escherichia coli work as Na + pumps. We have used an E. coli complex I preparation to determine the type of ion(s) translocated by means of enzyme activity, generation of a membrane potential and...