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Keywords: biophysics
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Biochem Soc Trans (2021) 49 (5): 2037–2050.
Published: 13 October 2021
.... Most membrane proteins are insoluble in aqueous solvent and embedded in the plasma membrane lipid bilayer, which significantly complicates biophysical studies. Poly(styrene-co-maleic acid) (SMA) and other polymer derivatives are increasingly common solubilisation agents, used to isolate membrane...
Biochem Soc Trans (2019) 47 (1): 63–76.
Published: 21 December 2018
... to further biophysical methods and to generalize it for binary molecular interactions. As seen in these examples, stringent validation is a very resource-intensive task and only possible where the two molecules involved in an interaction can directly be characterized. For all other cases, the validation...
Biochem Soc Trans (2018) 46 (2): 379–389.
Published: 14 March 2018
... vesicles. However, how the BAR proteins induce fission has not been well described. Biophysical simulation and in vitro liposome assays have shown that the N-BAR proteins endophilin and amphiphysin, which have 4 and 2 amphipathic helices, respectively, cause membrane fission dependent on the number...
Biochem Soc Trans (2014) 42 (2): 225–229.
Published: 20 March 2014
... biophysics energy landscape FoldIt protein folding Rosetta@home structure determination I was reminded by the citation for the Centenary Award of how much my research group's interests have changed since I started at the University of Washington in 1994. In the present article, I describe how...
Biochem Soc Trans (2010) 38 (6): 1587–1592.
Published: 24 November 2010
...-review, we introduce these quantitative fluorescence methods and discuss the impact they are beginning to have on the field. © The Authors Journal compilation © 2010 Biochemical Society 2010 biophysics eukaryotic ribosome Förster resonance energy transfer (FRET) translation initiation...
Biochem Soc Trans (2007) 35 (5): 966–969.
Published: 25 October 2007
...-stimulating proteins of p53 (ASPP) biophysics natively unfolded protein p53-mediated apoptosis proline-rich domain protein–protein interaction The tumour suppressor protein p53 is at the centre of a complex protein network, which provides one of the major anticancer defence mechanisms used...