... ]. In synthetic biology, research on protein synthesis inside synthetic compartments is now extensive [ 5 ]. Together with the optimization of the cell-free protein synthesis (CFPS), artificial cells allow the concealed knots in a translation stream to be revealed. Hence, creating artificial cells...

... by these methods can be hampered by difficulties in expressing the target proteins (e.g. insoluble, toxic or unstable proteins). Cell-free protein synthesis, which makes use of cell extracts to synthesize target proteins from added DNA, provides an alternative approach to expression that may overcome some...