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Keywords: cryo-electron microscopy
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Biochem Soc Trans (2024) BST20231553.
Published: 12 June 2024
...Isobel J. Hirst; William J.R. Thomas; Rhiannon A. Davies; Stephen P. Muench Sample preparation can present a significant hurdle within single particle cryo-electron microscopy (cryoEM), resulting in issues with reproducibility, data quality or an inability to visualise the sample. There are several...
Biochem Soc Trans (2023) 51 (4): 1533–1543.
Published: 10 August 2023
... a transformative agreement with Individual. cannabinoids cryo-electron microscopy crystallography G-protein-coupled receptors Human endocannabinoid signaling is primarily mediated by the class A G protein-coupled receptors (GPCRs) CB1 and CB2. Class A GPCRs consist of an extracellular N-terminal...
Biochem Soc Trans (2023) 51 (4): 1505–1520.
Published: 10 August 2023
... to switch movement directions [ 5 ]. Kinesin-13s are microtubule depolymerases and kinesin-8s combine plus end-directed motility and microtubule depolymerization activities [ 6 ]. cryo-electron microscopy crystallography kinesin microtubule molecular motors tubulin © 2023 The Author(s...
Includes: Supplementary data
Biochem Soc Trans (2023) 51 (3): 1225–1233.
Published: 04 May 2023
... for this article was enabled by the participation of the University of Geneva in an all-inclusive Read & Publish agreement with Portland Press and the Biochemical Society under a transformative agreement with Individual. cancer cell cycle cryo-electron microscopy enzyme–substrate interactions...
Biochem Soc Trans (2023) 51 (2): 855–869.
Published: 21 April 2023
... article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . caveolae caveolins cell membranes cryo-electron microscopy membrane proteins protein structure Nanoscale flask-shaped...
Biochem Soc Trans (2023) 51 (1): 87–99.
Published: 25 January 2023
... insights into how geometry and ABPs regulate the actin cytoskeleton limits our understanding of the molecular mechanisms that define actin cytoskeleton remodeling and, in turn, impact emerging cell migration characteristics. With the advent of cryo-electron microscopy (cryo-EM) and advanced computational...
Biochem Soc Trans (2023) 51 (1): 183–193.
Published: 20 January 2023
... by electron cryomicroscopy. Correspondence: John L. Rubinstein ( john.rubinstein@utoronto.ca ) 30 11 2022 3 1 2023 10 1 2023 © 2023 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2023 cryo-electron microscopy electron transport...
Biochem Soc Trans (2022) 50 (5): 1403–1414.
Published: 05 October 2022
.... cryo-electron microscopy molecular chaperones molecular mechanisms protein conformation A more recent study involving cryo-EM and hydrogen-deuterium exchange analysis of denatured actin added to purified CCT in different nucleotide binding states corroborated this model, showing unfolded...
Biochem Soc Trans (2021) 49 (6): 2711–2726.
Published: 02 December 2021
... invaluable, the ‘resolution revolution’ of cryo-electron microscopy has transformed our structural investigations, enabling large, dynamic and often transient transcription complexes to be resolved that in many cases had resisted crystallisation. In this review, we highlight the impact cryo-electron...
Biochem Soc Trans (2021) 49 (6): 2639–2653.
Published: 08 November 2021
... of the Biochemical Society 2021 chromatin cryo-electron microscopy epigenetics eukaryotic gene expression transcription Eukaryotic cells pack their DNA (∼6 billion bases) into the small volume of the nucleus (1 × 10 2 –1 × 10 5  µm 3 ) by organizing their DNA into complex but defined structures...
Biochem Soc Trans (2021) 49 (5): 2287–2298.
Published: 28 October 2021
...: Robert M. Glaeser ( rmglaeser@lbl.gov ) 6 9 2021 29 9 2021 5 10 2021 © 2021 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2021 cryo-electron microscopy divide and conquer structure determination All cameras...
Biochem Soc Trans (2021) 49 (5): 2345–2355.
Published: 28 September 2021
... will discuss the relative merits of cryo-EM and X-ray crystallography for the structure determination of GPCRs and the future potential of both techniques. cryo-electron microscopy crystallography G-protein-coupled receptors A significant advantage of cryo-EM over X-ray crystallography...
Biochem Soc Trans (2021) 49 (3): 1349–1359.
Published: 10 June 2021
... higher level of activity when purified within DIBMALPs than with detergent [ 60 ]. cryo-electron microscopy membrane proteins protein complexes protein–lipid interactions SMALP NMR based structural studies of membrane proteins can often be challenging simply due to their size, however...
Biochem Soc Trans (2020) 48 (5): 2261–2272.
Published: 14 October 2020
... biology, but recent advances in cryo-electron microscopy and cryo-electron tomography have further uncovered exciting new snapshots of reconstituted retromer structures. These new structures reveal retromer assembles into an arch-shaped scaffold and suggest the scaffold may be flexible and adaptable...
Biochem Soc Trans (2020) 48 (3): 1057–1066.
Published: 05 May 2020
... Society under a transformative agreement with JISC. biochemistry cryo-electron microscopy DNA replication in silico reconstitution single molecule Accurate transfer of genetic information from parental to daughter cells requires that chromosome replication is finely tuned, so that DNA...
Biochem Soc Trans (2019) 47 (1): 37–45.
Published: 15 January 2019
.... Biol. 25 , 616 – 622 10.1038/s41594-018-0085-x AAA proteins cryo-electron microscopy endosomal sorting protein disassembly protein structure E ndosomal S orting C omplex R equired for T ransport (ESCRT) pathways drive membrane fission in numerous cellular...
Biochem Soc Trans (2018) 46 (6): 1407–1422.
Published: 12 November 2018
...-resolution structural information that was mostly limited to individual proteins and smaller sub-complexes. Recent developments in the field of cryo-electron microscopy allowed the visualisation of fully assembled yeast and human spliceosomes, providing unprecedented insights into substrate recognition...
In Collection
Biochem Soc Trans (2018) 46 (4): 807–816.
Published: 22 June 2018
... that highlight the importance of structure determination of molecules embedded within their native environment, and propose future directions to improve CET performance and accessibility. cryo-electron microscopy cryo-electron tomography subtomogram averaging The electron microscope provides...
Biochem Soc Trans (2016) 44 (4): 1177–1182.
Published: 15 August 2016
... Press Limited on behalf of the Biochemical Society 2016 cryo-electron microscopy eukaryotic RNA polymerase complex integrated structural biology multiprotein assembly X-ray crystallography Our laboratory studies the mechanisms of gene transcription and its regulation in eukaryotic...
Biochem Soc Trans (2008) 36 (4): 677–683.
Published: 22 July 2008
...Robert J.C. Gilbert; Ian Brierley; John E.G. McCarthy High-resolution structures have given an extremely detailed view of aspects of ribosomes, including some near-functional states. Here, we review the importance of cryo-electron microscopy, among other techniques, in giving an understanding...
Biochem Soc Trans (2006) 34 (1): 1–6.
Published: 20 January 2006
... The Biochemical Society 2006 ADP-ribosylation cryo-electron microscopy diphthamide diphtheria toxin elongation factor 2 (EF2) exotoxin A In the elongation cycle of protein synthesis, elongation factor 1A delivers aminoacylated tRNA to the ribosomal A-site, whereafter the peptidyl transferase...