...Miles Graham; Peijun Zhang Developments in cryo-electron microscopy (cryo-EM) have been interwoven with the study of viruses ever since its first applications to biological systems. Following the success of single particle cryo-EM in the last decade, cryo-electron tomography (cryo-ET) is now...

... distance from the leading edge and, thus, filament age. actin cytoskeleton cell migration cryo-electron microscopy cryo-electron tomography Correspondence: Florian KM Schur ( [email protected] ) 1 12 2022 12 1 2023 12 1 2023 To obtain three...

... underlying chemotaxis behavior is mediated by large, highly ordered protein complexes known as chemosensory arrays. For nearly two decades, cryo-electron tomography (cryoET) has been used to image chemosensory arrays, providing an increasingly detailed understanding of their structure and function...

... architecture of many enveloped viruses pose a challenge for the more widely used structural biology techniques, such as X-ray crystallography. Cryo-electron tomography (cryo-ET), however, is a particularly well-suited tool for overcoming the limitations associated with visualizing the irregular shapes...

... and low contrast in the cryoET data because of the thickness of the intact bacterial cell. bacterial chemotaxis chemoreceptor array cryo-electron tomography cryoEM in-situ structure subtomogram averaging All motile bacteria and archaea examined to date possess a highly conserved...

...Joshua Hutchings; Giulia Zanetti Cryo-electron tomography (CET) is uniquely suited to obtain structural information from a wide range of biological scales, integrating and bridging knowledge from molecules to cells. In particular, CET can be used to visualise molecular structures in their native...

...Karen M. Davies; Bertram Daum To truly understand bioenergetic processes such as ATP synthesis, membrane-bound substrate transport or flagellar rotation, systems need to be analysed in a cellular context. Cryo-ET (cryo-electron tomography) is an essential part of this process, as it is currently...