... measurement of redox status in living cells. disulfide formation endoplasmic reticulum glutathione redox regulation thioredoxin Previously, roGFP probes have been used to study redox conditions within the cytosol [ 19 ], mitochondria [ 20 ] and later stages of the secretory pathway [ 21...

... that the inactivation of peroxiredoxins by hyperoxidation may allow H 2 O 2 levels to increase in cells and thereby modify critical thiol groups in proteins. We have been studying how the H 2 O 2 produced during disulfide formation in the ER (endoplasmic reticulum) is metabolized and have shown that ER-resident...