... To whom correspondence should be addressed (email [email protected] ). 29 4 2014 © The Authors Journal compilation © 2014 Biochemical Society 2014 co-localization single-molecule spectroscopy (CoSMoS) dynamics fluorescence resonance energy transfer (FRET) pre-mRNA splicing...

... is reported to decrease the sensitivity of a Ca 2+ sensor to low pH [ 27 ]. cAMP fluorescence resonance energy transfer (FRET) neuron pH mTFP1 (monomeric teal fluorescent protein 1) was developed, by directed evolution, from cFP484, a tetrameric CFP from Clavularia coral [ 24 ]. A synthetic...

... ). 3 9 2010 © The Authors Journal compilation © 2011 Biochemical Society 2011 ATP hydrolysis C-terminal domain DEAD-box fluorescence resonance energy transfer (FRET) RNA-binding domain RNA-recognition motif Many endergonic reactions in the cell are catalysed using the energy...

...-time. fluorescence resonance energy transfer (FRET) RNA polymerase TATA-box-binding protein (TBP) transcription RNAPs are the enzymes at the heart of transcription: they synthesize RNA in a DNA template-dependent manner [ 1 – 3 ]. RNAPs from all three domains of life are composed of 4–17...

... of ALEX to the study of protein–nucleic acid interactions. alternating-laser excitation (ALEX) biomolecular interaction fluorescence resonance energy transfer (FRET) protein–DNA interaction single-molecule fluorescence total internal reflection fluorescence (TIRF) Combination of ALEX...

... computational and experimental approach can yield. 1 To whom correspondence should be addressed (email [email protected] ). 18 9 2007 © The Authors Journal compilation © 2008 Biochemical Society 2008 computational chemistry fluorescence resonance energy transfer (FRET...

... the transition between early and late phagosomes. 1 To whom correspondence should be addressed, at The Hospital for Sick Children (email [email protected] ). 11 6 2007 © The Authors Journal compilation © 2007 Biochemical Society 2007 acidification fluorescence resonance energy transfer...

... that are markedly free from artefactual interferences. 1 email [email protected] 2 4 2007 © The Authors Journal compilation © 2007 Biochemical Society 2007 bioluminescence resonance energy transfer (BRET) enzyme fragment complementation (EFC) fluorescence resonance energy...

... technique. Molecular masses (M W ) are indicated in kDa. GF, gel filtration; IMAC, immobilized metal-ion-affinity chromatography. fluorescence resonance energy transfer (FRET) G-protein coupled receptor (GPCR) in-gel fluorescence neurotensin receptor type 1 (NTS1) solid-state NMR surface...

.... Alessi D.R. van Aalten D.M. EMBO J. 2004 23 3918 3928 Akt fluorescence resonance energy transfer (FRET) green fluorescent protein (GFP) imaging phosphoinositide 3-kinase (PI3K) Ral Small GTPases function as critical molecular switches in a variety of signal transduction...

... 2006 cAMP signalling cell imaging fluorescence resonance energy transfer (FRET) phosphodiesterase (PDE) restricted diffusion second messenger cAMP is a ubiquitous second messenger that mediates many extracellular signals in eukaryotes from yeast to human. Binding of hormones...

... interaction with other signalling pathways. adenylate cyclase (AC) calcium cAMP cyclic nucleotide-gated channel (CNGC) exchange protein directly activated by cAMP (Epac) fluorescence resonance energy transfer (FRET) phosphodiesterase (PDE) Numerous hormones and neurotransmitters, acting...

... © 2005 The Biochemical Society 2005 biosensor cAMP cell imaging fluorescence resonance energy transfer (FRET) protein kinase A signal transduction cAMP is a ubiquitous second messenger that mediates the action of various hormones and neurotransmitters and influences many cell...

... 27 Sarner S. Kozma R. Ahmed S. Lim L. Mol. Cell. Biol. 2000 20 158 172 Cdc42 fluorescence resonance energy transfer (FRET) GTPase-activating protein (GAP) imaging Rac Rho Rho-family GTPases function as critical molecular switches in transducing...

... fluorescence resonance energy transfer (FRET) hydrolysis single-molecule fluorescence subunit rotation The H + -ATP synthase from Escherichia coli is a large membrane-bound enzyme that consists of eight different subunits. These subunits comprise two parts: the hydrophilic F 1 part with subunit...

.... Smrcka A.V. Wu G. Li L. Liu M. Cell (Cambridge, Mass.) 2003 114 215 227 17 Lippincott-Schwartz J. Snapp E. Kenworthy A. Nat. Rev. Mol. Cell Biol. 2001 2 444 456 actin cytoskeleton Cdc42 fluorescence resonance energy transfer (FRET) motility p21...

... To whom correspondence should be addressed (email [email protected] ). 17 1 2005 © 2005 The Biochemical Society 2005 acid–base catalysis fluorescence resonance energy transfer (FRET) hairpin ribozyme RNA catalysis single-molecule spectroscopy The hairpin ribozyme...

... provided us with the possibility of studying metabolic processes in living organisms. However, at present these technologies do not permit measuring at the subcellular level. The cameleon , a fluorescence resonance energy transfer (FRET)-based nanosensor uses the ability of the calcium-bound form...

... ). 23 6 2004 © 2004 The Biochemical Society 2004 endoplasmic reticulum membrane fluorescence spectroscopy fluorescence resonance energy transfer (FRET) nascent membrane protein ribosome translocon Not long after ribosomes had been identified and shown to be responsible...

.... Jacobson K. Science 2002 296 1821 1825 1 To whom correspondence should be addressed (email [email protected] ). 8 7 2004 © 2004 The Biochemical Society 2004 clustering fluorescence resonance energy transfer (FRET) glycosylphosphatidylinositol (GPI)-anchored...

... it is possible that GRK phosphorylation of GPCRs depends on an additional conformational change in the receptor, which is not required for either G-protein activation or arrestin binding. © 2004 The Biochemical Society 2004 arrestin fluorescence resonance energy transfer (FRET) G-protein-coupled...

...), fluorescence resonance energy transfer (FRET), signalling. Abbreviations used: FLIM, fluorescence lifetime imaging microscopy; FRET, fluorescence resonance energy transfer; GFP, green fluorescent protein; PAK1, p21-activated kinase-1; PKC, protein kinase C; RFP, red fluorescent protein. 1To whom correspondence...

... Chemistry and Biology: The 5th Cambridge Symposium, a Biochemical Society-sponsored meeting held at Queen's College, Cambridge, 31 August–3 September 2003 3 September 2003 © 2004 Biochemical Society 2004 branch migration fluorescence resonance energy transfer (FRET) hairpin ribozyme...

...-uptake mechanism involves all the proteins Key words: fluorescence resonance energy transfer (FRET), iron uptake, outer membrane protein, siderophore. Abbreviations used: Kdapp, apparent dissociation constant; PaA. pyoverd in. 'To whom comsDondence should be addressed (e-mail generally reported...