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Keywords: fluorescence resonance energy transfer (FRET)
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Articles
Biochem Soc Trans (2014) 42 (4): 1211–1218.
Published: 11 August 2014
... ). 29 4 2014 © The Authors Journal compilation © 2014 Biochemical Society 2014 co-localization single-molecule spectroscopy (CoSMoS) dynamics fluorescence resonance energy transfer (FRET) pre-mRNA splicing single-molecule fluorescence splicessomal RNA Splicing plays a major...
Articles
Biochem Soc Trans (2012) 40 (1): 179–183.
Published: 19 January 2012
... cAMP fluorescence resonance energy transfer (FRET) neuron pH Depolarization of excitable cells leads to Ca 2+ influx and significant decreases in intracellular pH [ 10 ]. These changes in pH can be attributed to the actions of the PMCA (plasma membrane Ca 2+ -ATPase) Ca 2+ /H + exchange...
Articles
Biochem Soc Trans (2011) 39 (2): 611–616.
Published: 22 March 2011
... ). 3 9 2010 © The Authors Journal compilation © 2011 Biochemical Society 2011 ATP hydrolysis C-terminal domain DEAD-box fluorescence resonance energy transfer (FRET) RNA-binding domain RNA-recognition motif Many endergonic reactions in the cell are catalysed using the energy...
Articles
Biochem Soc Trans (2011) 39 (1): 122–127.
Published: 19 January 2011
...-time. 1 To whom correspondence should be addressed (email d.grohmann@ucl.ac.uk ). 9 11 2010 © The Authors Journal compilation © 2011 Biochemical Society 2011 fluorescence resonance energy transfer (FRET) RNA polymerase TATA-box-binding protein (TBP) transcription...
Articles
Biochem Soc Trans (2008) 36 (4): 738–744.
Published: 22 July 2008
... of ALEX to the study of protein–nucleic acid interactions. alternating-laser excitation (ALEX) biomolecular interaction fluorescence resonance energy transfer (FRET) protein–DNA interaction single-molecule fluorescence total internal reflection fluorescence (TIRF) The last 15 years...
Articles
Biochem Soc Trans (2008) 36 (1): 46–50.
Published: 22 January 2008
... computational and experimental approach can yield. 1 To whom correspondence should be addressed (email richard.bryce@manchester.ac.uk ). 18 9 2007 © The Authors Journal compilation © 2008 Biochemical Society 2008 computational chemistry fluorescence resonance energy transfer (FRET...
Articles
Biochem Soc Trans (2007) 35 (5): 1083–1087.
Published: 25 October 2007
... the transition between early and late phagosomes. 1 To whom correspondence should be addressed, at The Hospital for Sick Children (email sga@sickkids.ca ). 11 6 2007 © The Authors Journal compilation © 2007 Biochemical Society 2007 acidification fluorescence resonance energy transfer...
Articles
Biochem Soc Trans (2007) 35 (4): 746–748.
Published: 20 July 2007
... fragment complementation (EFC) fluorescence resonance energy transfer (FRET) G-protein-coupled receptor screening (GPCR screening) protein complementation Historically, evaluation of large numbers of compounds at GPCRs employs highly automated liquid-dispensing systems, accompanied by detection...
Articles
Biochem Soc Trans (2007) 35 (4): 760–763.
Published: 20 July 2007
...; IMAC, immobilized metal-ion-affinity chromatography. fluorescence resonance energy transfer (FRET) G-protein coupled receptor (GPCR) in-gel fluorescence neurotensin receptor type 1 (NTS1) solid-state NMR surface plasmon resonance GPCRs (G-protein coupled receptors) are a family...
Articles
Biochem Soc Trans (2006) 34 (5): 851–854.
Published: 25 October 2006
... protrusion; this suggests that the recruitment of Ral GEFs to the plasma membrane is not sufficient for Ral activation. Akt fluorescence resonance energy transfer (FRET) green fluorescent protein (GFP) imaging phosphoinositide 3-kinase (PI3K) Ral Small GTPases function as critical molecular...
Articles
Biochem Soc Trans (2006) 34 (4): 495–497.
Published: 21 July 2006
... 2006 cAMP signalling cell imaging fluorescence resonance energy transfer (FRET) phosphodiesterase (PDE) restricted diffusion second messenger cAMP is a ubiquitous second messenger that mediates many extracellular signals in eukaryotes from yeast to human. Binding of hormones...
Articles
Biochem Soc Trans (2006) 34 (4): 468–471.
Published: 21 July 2006
... interaction with other signalling pathways. adenylate cyclase (AC) calcium cAMP cyclic nucleotide-gated channel (CNGC) exchange protein directly activated by cAMP (Epac) fluorescence resonance energy transfer (FRET) phosphodiesterase (PDE) Numerous hormones and neurotransmitters, acting...
Articles
Biochem Soc Trans (2005) 33 (6): 1323–1326.
Published: 26 October 2005
... The Biochemical Society 2005 biosensor cAMP cell imaging fluorescence resonance energy transfer (FRET) protein kinase A signal transduction cAMP is a ubiquitous second messenger that mediates the action of various hormones and neurotransmitters and influences many cell functions by cross-talk...
Articles
Biochem Soc Trans (2005) 33 (4): 878–882.
Published: 01 August 2005
... fluorescence resonance energy transfer (FRET) hydrolysis single-molecule fluorescence subunit rotation The H + -ATP synthase from Escherichia coli is a large membrane-bound enzyme that consists of eight different subunits. These subunits comprise two parts: the hydrophilic F 1 part with subunit...
Articles
Biochem Soc Trans (2005) 33 (4): 631–634.
Published: 01 August 2005
... of the role of Rho-family GTPases in the cell structure. Cdc42 fluorescence resonance energy transfer (FRET) GTPase-activating protein (GAP) imaging Rac Rho Rho-family GTPases function as critical molecular switches in transducing extracellular signals to the cytoskeleton [ 1 , 2...
Articles
Biochem Soc Trans (2005) 33 (4): 646–648.
Published: 01 August 2005
... resonance energy transfer (FRET) motility p21-activated kinase (Pak) Rac1 Pak1 (p21-activated kinase 1) is the first identified and most studied member of the Pak family comprising six serine/threonine protein kinases in humans. Pak family members present structural similarities...
Articles
Biochem Soc Trans (2005) 33 (3): 461–465.
Published: 01 June 2005
... fluorescence resonance energy transfer (FRET) hairpin ribozyme RNA catalysis single-molecule spectroscopy © 2005 The Biochemical Society 2005 The same cross-correlation analysis was applied to the E app data for the low-FRET state of the complete ribozyme, whereupon it was found that a similar...
Articles
Biochem Soc Trans (2005) 33 (1): 287–290.
Published: 01 February 2005
... provided us with the possibility of studying metabolic processes in living organisms. However, at present these technologies do not permit measuring at the subcellular level. The cameleon , a fluorescence resonance energy transfer (FRET)-based nanosensor uses the ability of the calcium-bound form...
Articles
Biochem Soc Trans (2004) 32 (5): 668–672.
Published: 26 October 2004
...@tamu.edu ). 23 6 2004 © 2004 The Biochemical Society 2004 endoplasmic reticulum membrane fluorescence spectroscopy fluorescence resonance energy transfer (FRET) nascent membrane protein ribosome translocon Not long after ribosomes had been identified and shown...
Articles
Biochem Soc Trans (2004) 32 (5): 673–675.
Published: 26 October 2004
... membrane convolution. Scale bar, 5 μm. 1 To whom correspondence should be addressed (email og@mrc-lmb.cam.ac.uk ). 8 7 2004 © 2004 The Biochemical Society 2004 clustering fluorescence resonance energy transfer (FRET) glycosylphosphatidylinositol (GPI)-anchored proteins...
Articles
Biochem Soc Trans (2004) 32 (6): 1029–1031.
Published: 26 October 2004
... is not required for either G-protein activation or arrestin binding. © 2004 The Biochemical Society 2004 arrestin fluorescence resonance energy transfer (FRET) G-protein-coupled receptor (GPCR) heterotrimeric G-protein kinetics ...
Articles
Biochem Soc Trans (2004) 32 (3): 431–433.
Published: 01 June 2004
... of Integrins 431 Imaging protein protein interactions in cell motility using fluorescence resonance energy transfer (FRET) M. Parsons*1, B. Vojnovic and S. Ameer-Beg *Randall Centre, New Hunts House, Kings College London, Guys Campus, London SE1 1UL, U.K., and Gray Cancer Institute, P.O. Box 100, Mount...
Articles
Biochem Soc Trans (2004) 32 (1): 41–45.
Published: 01 February 2004
... Chemistry and Biology: The 5th Cambridge Symposium, a Biochemical Society-sponsored meeting held at Queen's College, Cambridge, 31 August–3 September 2003 3 September 2003 © 2004 Biochemical Society 2004 branch migration fluorescence resonance energy transfer (FRET) hairpin ribozyme...
Articles
Biochem Soc Trans (2002) 30 (4): 702–705.
Published: 01 August 2002
... © 2002 Biochemical Society 2002 fluorescence resonance energy transfer (FRET) iron uptake outer membrane protein siderophore Biometals 2002: Third International Biometals Symposium: Focused Meeting Organized by S. Andrews (School of Animal and Microbial Sciences, University of Reading...