... for two emerging concepts that affect insulin secretion at the level of individual granules: (i) the existence of specialized sites where granules dock in preparation for exocytosis; and (ii) post-exocytotic regulation of cargo release by the fusion pore. 1 To whom correspondence should...

... evanescent wave microscopy exocytosis fusion pore kiss-and-run synaptotagmin Abbreviations used: (E)GFP, (enhanced) green fluorescent protein; NPY, neuropeptide Y; pHluorin, pH-sensitive enhanced green fluorescent protein; phogrin, pho sphatase on the gr anule of in sulinoma...

... of typical mast cell granules. We have measured the time course of fusion pore expansion, and correlated it with release from the granule matrix. The fusion pore of mast cell granules grows in three stages. The initial expansion of the pore occurred at a rate of 5 nS/s, and in many cases an observable...