...Daniel B. Williamson; Robert S. Haltiwanger O -glycosylation of Epidermal Growth Factor-like (EGF) repeats plays crucial roles in protein folding, trafficking and function. The Notch extracellular domain has been used as a model to study these mechanisms due to its many O- glycosylated EGF repeats...

...- GlcNAc on specific protein substrates. To interrogate the functional roles of O- GlcNAc on a desired target protein, producing highly glycosylated or deglycosylated target proteins is necessary. In general, incubation of a purified target protein with OGT in vitro readily affords high O...

... reporter chemoenzymatic labelling glycobiology glycosylation metabolic engineering Glycans play crucial roles in a diversity of cellular processes. For example, glycoproteins are essential mediators of cell–cell and cell–matrix interactions, they are involved in cell recognition and signalling...

...Roberto Donini; Stuart M. Haslam; Cleo Kontoravdi Biotherapeutic glycoproteins have revolutionised the field of pharmaceuticals, with new discoveries and continuous improvements underpinning the rapid growth of this industry. N-glycosylation is a critical quality attribute of biotherapeutic...

...Joana G. Rodrigues; Henrique O. Duarte; Celso A. Reis; Joana Gomes Aberrant cell surface glycosylation signatures are currently known to actively drive the neoplastic transformation of healthy cells. By disrupting the homeostatic functions of their protein carriers, cancer-associated glycans...

... glycoproteins glycoproteomics glycosylation LC-MS/MS mass spectrometry The human genome encodes a plethora of enzymes, receptors, and transporters that concertedly facilitate and regulate protein glycosylation, the covalent attachment of complex carbohydrates (glycans) to polypeptides. Glycosylation...

... article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . biosynthesis glycosylation promiscuity The biosynthesis of several physiologically important small molecules and oligosaccharides...

...-glycosylation pathways to virus replication and the drugs available to disrupt these pathways. In particular, we make a case for evaluation of the well-tolerated drugs miglitol, celgosivir and especially miglustat for the treatment of COVID-19. Correspondence: Ethan D. Goddard-Borger ( Goddard-Borger.E...

... lectin receptors. These lectins may contribute to initial recognition of bacterial glycans, thus providing an early defence mechanism against bacterial infections, but they may also be exploited by bacteria to escape immune responses. In this review, we will first exemplify bacterial glycosylation...

... 30 disulfide bonds and around a 100 N-linked glycosylation sites, and are functionally dependent on protease cleavage within the secretory system. The resulting mature structure adopts a compact fold with the vast majority of its surface obscured by a protective shield of glycans which can...

...Maria C. Rodriguez Benavente; Pablo Argüeso Glycosylation is a major form of enzymatic modification of organic molecules responsible for multiple biological processes in an organism. The biosynthesis of glycans is controlled by a series of glycosyltransferases, glycosidases and glycan-modifying...

... by Portland Press Limited on behalf of the Biochemical Society 2017 adhesins gastrointestinal mucins glycosylation microbiota pathogens The number of bacteria constituting the human microbiota is nearly the same as the number of cells in the human body and most of these microorganisms...

...Jayne E. Telford; Margaret A. Doherty; Tharmala Tharmalingam; Pauline M. Rudd Glycosylation modifications have been reported in a number of disease states and, as a result, there is significant focus on the discovery and development of glycan-based biomarkers. Glyco-biomarkers have the potential...

...Jane E. Hewitt During the last 10 years it has become apparent that a significant subset of inherited muscular dystrophy is caused by errors in the glycosylation of α-dystroglycan. Many of these dystrophies are also associated with abnormalities of the central nervous system. Dystroglycan has...

.... Aberrant glycosylation is a hallmark of malignancy and a well-studied class of β1,6-branched oligosaccharides is associated with malignant transformation of rodent and human cells, and poor prognosis in cancer patients. It is evident that increased β1,6 branching significantly contributes to the phenotype...

... substitutions. What poses an enormous challenge for analytical processing is, at the same time, the basis for the fingerprint-like glycomic profiles of glycoconjugates and cells. What's more, the glycosylation machinery is sensitive to disease manifestations, earning glycan assembly a reputation as a promising...

...Eirikur Saeland; Yvette van Kooyk A common phenotypic change in cancer is a dramatic transformation of cellular glycosylation. Functional studies of particular tumour-associated oligosaccharides are difficult to interpret conclusively, but carbohydrate-binding proteins are likely to contribute...

...Li Liu; Jayne E. Telford; Ana Knezevic; Pauline M. Rudd The development of glycoanalytical HPLC-based high-throughput technology has greatly enhanced the study of glycobiology, facilitating the discovery of disease-related solutions and providing an informative view of glycosylation and its...

...Paul G. Hitchen; Katie Twigger; Esmeralda Valiente; Rebecca H. Langdon; Brendan W. Wren; Anne Dell With glycosylation now firmly established across both Archaeal and bacterial proteins, a wide array of glycan diversity has become evident from structural analysis and genomic data. These discoveries...

... to ensure translation of sugar signals into an array of biological responses. This process of a lectin–carbohydrate rendezvous is likely to be intimately regulated. Analysing this interaction closely discerns six levels of affinity regulation. glycoprotein glycosylation inflammation lectin...

... of PrP's N-glycans to the misfolding of the protein. However, despite the absence of spontaneous PrP misfolding in these models, all our glycosylation-deficient lines are susceptible to transmitted TSE and accumulate PrP Sc during the disease process. The lack of spontaneous formation of PrP Sc in our...

... 2004 Alzheimer's disease amyloidogenesis cholesterol glycosylation lovastatin proteolysis β-secretase Abbreviations used: Aβ, β-amyloid; APP, Alzheimer's amyloid precursor protein; APPswe, Swedish mutant of APP; ASP-2, aspartic protease 2; FCS, fetal calf serum; Endo H...