...-GlcNAc levels in diabetes is lacking. 21 8 2024 23 10 2024 29 10 2024 © 2024 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2024 diabetes glycosylation myocardium O-GlcNAc The increased risk of heart failure associated...

...Daniel B. Williamson; Robert S. Haltiwanger O -glycosylation of Epidermal Growth Factor-like (EGF) repeats plays crucial roles in protein folding, trafficking and function. The Notch extracellular domain has been used as a model to study these mechanisms due to its many O- glycosylated EGF repeats...

... deglycosylation glycosylation O -GlcNAc post translational modification protein engineering O- GlcNAc is a monosaccharide post-translational modification (PTM) installed on thousands of nucleocytoplasmic and mitochondrial proteins at multiple serine or threonine residues in eukaryotic cells [ 1...

... in these areas, in combination with a growing number of bioorthogonal ligation reactions [ 8 , 20 ], have greatly expanded our glycan labelling toolkit. In this mini-review, we will discuss key concepts of MOE and CeGL strategies, focusing on their application to the study of mammalian O-glycosylation...

...Roberto Donini; Stuart M. Haslam; Cleo Kontoravdi Biotherapeutic glycoproteins have revolutionised the field of pharmaceuticals, with new discoveries and continuous improvements underpinning the rapid growth of this industry. N-glycosylation is a critical quality attribute of biotherapeutic...

...Joana G. Rodrigues; Henrique O. Duarte; Celso A. Reis; Joana Gomes Aberrant cell surface glycosylation signatures are currently known to actively drive the neoplastic transformation of healthy cells. By disrupting the homeostatic functions of their protein carriers, cancer-associated glycans...

... in the immediate future. Dysregulation of the glycosylation machinery often leads to aberrant protein glycosylation. Altered glycophenotypes can arise from and, in other cases, lead to a broad range of pathologies, amongst others, cancer [ 11–13 ], infection and inflammation [ 14–17 ], and schizophrenia [ 18...

... glycosylation, is carried out by specialized enzymes known as glycosyltransferases (GTases) [ 1 ]. GTases catalyze glycosidic linkages between monosaccharides on a donor substrate (usually a nucleotide-linked sugar) and a moiety on an acceptor substrate (a small molecule or oligosaccharide) ( Figure 1A ). Many...

...-glycosylation pathways to virus replication and the drugs available to disrupt these pathways. In particular, we make a case for evaluation of the well-tolerated drugs miglitol, celgosivir and especially miglustat for the treatment of COVID-19. © 2020 The Author(s) 2020 This is an open access article...

... lectin receptors. These lectins may contribute to initial recognition of bacterial glycans, thus providing an early defence mechanism against bacterial infections, but they may also be exploited by bacteria to escape immune responses. In this review, we will first exemplify bacterial glycosylation...

... 30 disulfide bonds and around a 100 N-linked glycosylation sites, and are functionally dependent on protease cleavage within the secretory system. The resulting mature structure adopts a compact fold with the vast majority of its surface obscured by a protective shield of glycans which can...

...Maria C. Rodriguez Benavente; Pablo Argüeso Glycosylation is a major form of enzymatic modification of organic molecules responsible for multiple biological processes in an organism. The biosynthesis of glycans is controlled by a series of glycosyltransferases, glycosidases and glycan-modifying...

... the gastrointestinal tract, being thickest in the stomach and the colon compared with the small intestine. In humans, for example, adherent colonic mucus is thought to measure ∼150 µm [ 3 ]. adhesins gastrointestinal mucins glycosylation microbiota pathogens The number of bacteria constituting...

...Jayne E. Telford; Margaret A. Doherty; Tharmala Tharmalingam; Pauline M. Rudd Glycosylation modifications have been reported in a number of disease states and, as a result, there is significant focus on the discovery and development of glycan-based biomarkers. Glyco-biomarkers have the potential...

...Jane E. Hewitt During the last 10 years it has become apparent that a significant subset of inherited muscular dystrophy is caused by errors in the glycosylation of α-dystroglycan. Many of these dystrophies are also associated with abnormalities of the central nervous system. Dystroglycan has...

.... Aberrant glycosylation is a hallmark of malignancy and a well-studied class of β1,6-branched oligosaccharides is associated with malignant transformation of rodent and human cells, and poor prognosis in cancer patients. It is evident that increased β1,6 branching significantly contributes to the phenotype...

...Eirikur Saeland; Yvette van Kooyk A common phenotypic change in cancer is a dramatic transformation of cellular glycosylation. Functional studies of particular tumour-associated oligosaccharides are difficult to interpret conclusively, but carbohydrate-binding proteins are likely to contribute...

... substitutions. What poses an enormous challenge for analytical processing is, at the same time, the basis for the fingerprint-like glycomic profiles of glycoconjugates and cells. What's more, the glycosylation machinery is sensitive to disease manifestations, earning glycan assembly a reputation as a promising...

...Li Liu; Jayne E. Telford; Ana Knezevic; Pauline M. Rudd The development of glycoanalytical HPLC-based high-throughput technology has greatly enhanced the study of glycobiology, facilitating the discovery of disease-related solutions and providing an informative view of glycosylation and its...

...Paul G. Hitchen; Katie Twigger; Esmeralda Valiente; Rebecca H. Langdon; Brendan W. Wren; Anne Dell With glycosylation now firmly established across both Archaeal and bacterial proteins, a wide array of glycan diversity has become evident from structural analysis and genomic data. These discoveries...

.... Schachter H. Gabius H.-J. Gabius S. Glycosyltransferases involved in N- and O-glycan biosynthesis Glycosciences: Status and Perspectives 1997 London Chapman and Hall 79 113 6 Reuter G. Gabius H.-J. Eukaryotic glycosylation: whim of nature or multipurpose tool? Cell. Mol...

... correspondence should be addressed (email [email protected] ). 1 7 2005 © 2005 The Biochemical Society 2005 Creutzfeldt–Jacob disease (CJD) glycosylation prion protein folding PrP transmissible spongiform encephalopathy (TSE) Prusiner first proposed the prion...

... 2004 Alzheimer's disease amyloidogenesis cholesterol glycosylation lovastatin proteolysis β-secretase Abbreviations used: Aβ, β-amyloid; APP, Alzheimer's amyloid precursor protein; APPswe, Swedish mutant of APP; ASP-2, aspartic protease 2; FCS, fetal calf serum; Endo H...