... organism but conventional techniques to quantify aging biomarkers are laborious and resource-intensive. High-throughput screening (HTS) methods offer a solution to this challenge by facilitating high-order data collection from large sample populations. These approaches involve the use of (i) large-scale...

... genomics high-throughput screening induced pluripotent stem cells neurodegeneration Parkinson's disease In addition, CRISPRi may be used as a tool for the validation of differentially regulated genes/proteins or pathways identified by -omics approaches or to recapitulate-omics signatures...

... article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . biological networks genomics high-throughput screening A principal goal of many phenomics projects is to shed light on the complex...

... targets high-throughput screening large-scale protein-fragment complementation assays protein–protein interactions proteome-wide analysis Protein–protein interactions (PPIs) constitute a complex cellular network and play a central role in nearly all biological processes, including DNA...

... for high-throughput screenings. binder proteins crystallography high-throughput screening recombinant proteins Stability. Thermal stability of the sample protein is often assessed with T m upon thermal denaturation under various conditions. A large-scale analysis of the thermal...

... screening. We also discuss the challenge to achieve a safe and targeted delivery of miRNA therapeutics in cardiovascular cells. cardiovascular disease gene therapy high-throughput screening microRNA Correspondence: Andrea Caporali ( [email protected] ) To identify miRNAs that alter...

...-based high-throughput screen assay for leucine-rich repeat kinase 2 — discovery of LRRK2 inhibitors . Anal. Biochem. 404 , 186 – 192 doi: 10.1016/j.ab.2010.05.033 11 Zhang , J. , Deng , X. , Choi , H.G. , Alessi , D.R. and Gray , N.S. ( 2012 ) Characterization...

... sites and their regulation can be adapted to high-throughput-screening-amenable platforms. The present review describes the utilization of LRRK2 phosphorylation as indicators of enzyme inhibition, as well as how such assays can be used to deconvolute the pathways in which LRRK2 plays a role. 1...

... potential for their use in regenerative therapy, the challenge remains to understand the basic biology of these remarkable cells, in order to differentiate them into any functional cell type. Given the scale of the task, high-throughput screening of agents and culture conditions offers one way to accelerate...

... to HRP is then added. HRP substrate is added after a final wash step. 1 To whom correspondence should be addressed (email [email protected] ). 15 5 2009 © The Authors Journal compilation © 2010 Biochemical Society 2010 high-throughput screening homologous with E6...

... Factor X/Xa fluorescence polarization high-throughput screening New Approaches for Elucidating Protease Biology and Therapeutic Opportunities: Biochemical Society Focused Meeting held at Royal Agricultural College, Cirencester, U.K., 4–6 January 2007. Organized by B. Austen (St George's...

... (1988) J. Biol. Chem. 263 , 8177–8182], an example where binding does not involve a secondary structural change. Synchrotron radiation CD spectroscopy offers significant enhancements with respect to conventional CD spectroscopy, which will enable its usage for high-throughput screening and as a tool...

...A. Clark; S. Howell 1 To whom correspondence should be addressed(e-mail [email protected] ) 9 4 2002 © 2002 Biochemical Society 2002 high-throughput screening ion channel nuclear receptor HTS, high-throughput screening High-Throughput Screening: The Way...

...J. H. Connick The high-throughput screening platform implemented for drug discovery is driven by the therapeutic areas of interest. Therefore the speed and information derived is governed by these areas. Multiple technologies are needed to exploit this and it is also important to show reactivity...