Search Results | Biochemical Society Transactions

Skip Nav Destination

1-34 of 34

Sort by

Sort Order Select

Articles

The energetics of protein–lipid interactions as viewed by molecular simulations

Robin A. Corey, Phillip J. Stansfeld, Mark S.P. Sansom

Biochem Soc Trans (2020) 48 (1): 25–37.

DOI: https://doi.org/10.1042/BST20190149

Published: 24 December 2019

...Robin A. Corey; Phillip J. Stansfeld; Mark S.P. Sansom Membranes are formed from a bilayer containing diverse lipid species with which membrane proteins interact. Integral, membrane proteins are embedded in this bilayer, where they interact with lipids from their surroundings, whilst peripheral...

View article titled, The energetics of protein–lipid interactions as viewed by molecular simulations

Articles

Crystallizing the function of the magnetosome membrane mineralization protein Mms6

Sarah S. Staniland, Andrea E. Rawlings

Biochem Soc Trans (2016) 44 (3): 883–890.

DOI: https://doi.org/10.1042/BST20160057

Published: 09 June 2016

... The Author(s) 2016 This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution Licence 4.0 (CC BY) . iron-binding protein magnetite nucleation magnetosome magnetotactic bacteria membrane protein...

View article titled, Crystallizing the function of the magnetosome membrane mineralization protein Mms6

Articles

Preparation of purified GPCRs for structural studies

Robert M. Cooke, Markus Koglin, James C. Errey, Fiona H. Marshall

Biochem Soc Trans (2013) 41 (1): 185–190.

DOI: https://doi.org/10.1042/BST20120240

Published: 29 January 2013

... © The Authors Journal compilation © 2013 Biochemical Society 2013 detergent G-protein-coupled receptor membrane protein thermostability Determining the structures of proteins by X-ray diffraction relies on generating milligram quantities of pure, correctly folded material, which can...

View article titled, Preparation of purified GPCRs for structural studies

Articles

The quest to achieve the detailed structural and functional characterization of CymA

Ricardo O. Louro, Catarina M. Paquete

Biochem Soc Trans (2012) 40 (6): 1291–1294.

DOI: https://doi.org/10.1042/BST20120114

Published: 21 November 2012

... and functional mechanisms of this focal protein. Understanding the structure and function of membrane proteins is hampered by inherent difficulties associated with their purification since the choice of the detergents play a critical role in the protein structure and stability. In the present mini-review, we...

View article titled, The quest to achieve the detailed structural and functional characterization of CymA

Articles

Membrane proteins: from bench to bits

Gunnar von Heijne

Biochem Soc Trans (2011) 39 (3): 747–750.

DOI: https://doi.org/10.1042/BST0390747

Published: 20 May 2011

...Gunnar von Heijne Membrane proteins currently receive a lot of attention, in large part thanks to a steady stream of high-resolution X-ray structures. Although the first few structures showed proteins composed of tightly packed bundles of very hydrophobic more or less straight transmembrane α...

View article titled, Membrane proteins: from bench to bits

Articles

Lipid–protein interactions

Anthony G. Lee

Biochem Soc Trans (2011) 39 (3): 761–766.

DOI: https://doi.org/10.1042/BST0390761

Published: 20 May 2011

...Anthony G. Lee Intrinsic membrane proteins are solvated by a shell of lipid molecules interacting with the membrane-penetrating surface of the protein; these lipid molecules are referred to as annular lipids. Lipid molecules are also found bound between transmembrane α-helices; these are referred...

View article titled, Lipid–protein interactions

Articles

Surfactant-free purification of membrane proteins with intact native membrane environment

Mohammed Jamshad, Yu-Pin Lin, Timothy J. Knowles, Rosemary A. Parslow, Craig Harris, Mark Wheatley, David R. Poyner, Roslyn M. Bill, Owen R.T. Thomas, Michael Overduin, Tim R. Dafforn

Biochem Soc Trans (2011) 39 (3): 813–818.

DOI: https://doi.org/10.1042/BST0390813

Published: 20 May 2011

... is purified away from all others. For soluble proteins, this process is greatly aided by the lack of any restriction on the free and independent diffusion of individual protein particles in three dimensions. This is not the case for membrane proteins, as the membrane itself forms a continuum that joins...

View article titled, Surfactant-free purification of membrane proteins with intact native membrane environment

Articles

Unravelling the folding and stability of an ABC (ATP-binding cassette) transporter

Natalie DiBartolo, Paula J. Booth

Biochem Soc Trans (2011) 39 (3): 751–760.

DOI: https://doi.org/10.1042/BST0390751

Published: 20 May 2011

... of the intersubunit interactions of membrane protein complexes that contain both hydrophobic and hydrophilic subunits. In the present paper, we focus on the vitamin B 12 importer of Escherichia coli , BtuCD, that contains two transmembrane BtuC subunits and two ATP-binding BtuD subunits. We have studied the factors...

View article titled, Unravelling the folding and stability of an ABC (ATP-binding cassette) transporter

Articles

Lipid–protein interactions as determinants of membrane protein structure and function

William Dowhan, Mikhail Bogdanov

Biochem Soc Trans (2011) 39 (3): 767–774.

DOI: https://doi.org/10.1042/BST0390767

Published: 20 May 2011

...William Dowhan; Mikhail Bogdanov To determine how the lipid environment affects membrane protein structure and function, strains of Escherichia coli were developed in which normal phospholipid composition can be altered or foreign lipids can be introduced. The properties of LacY (lactose permease...

View article titled, Lipid–protein interactions as determinants of membrane protein structure and function

Articles

Endogenous ion channel complexes: the NMDA receptor

René A.W. Frank

Biochem Soc Trans (2011) 39 (3): 707–718.

DOI: https://doi.org/10.1042/BST0390707

Published: 20 May 2011

... 18 3 2011 © The Authors Journal compilation © 2011 Biochemical Society 2011 glutamate receptor hetero-oligomeric protein ionotropic receptor membrane protein N -methyl- D -aspartate receptor (NMDA receptor) tandem affinity purification-tagged mouse (TAP-tagged mouse...

View article titled, Endogenous ion channel complexes: the NMDA receptor

Articles

Membrane proteins in nanotechnology

Paul Curnow

Biochem Soc Trans (2009) 37 (4): 643–652.

DOI: https://doi.org/10.1042/BST0370643

Published: 22 July 2009

...Paul Curnow Integral membrane proteins are important biological macromolecules with structural features and functionalities that make them attractive targets for nanotechnology. I provide here a broad review of current activity in nanotechnology related to membrane proteins, including...

View article titled, Membrane proteins in nanotechnology

Articles

The bacterial respiratory nitric oxide reductase

Nicholas J. Watmough, Sarah J. Field, Ross J. L. Hughes, David J. Richardson

Biochem Soc Trans (2009) 37 (2): 392–399.

DOI: https://doi.org/10.1042/BST0370392

Published: 20 March 2009

... Biochemical Society 2009 electron transfer haem-copper oxidase membrane protein nitric oxide nitrous oxide proton transfer Movement of protons to the dinuclear centre of HCuOs, which lies buried in the hydrophobic interior of the membrane, is via pathways formed by ionizable amino acid side...

View article titled, The bacterial respiratory nitric oxide reductase

Articles

The petite purple photosynthetic powerpack

Michael R. Jones

Biochem Soc Trans (2009) 37 (2): 400–407.

DOI: https://doi.org/10.1042/BST0370400

Published: 20 March 2009

... of the structure and mechanism of the purple bacterial RC, focusing in the main on the most heavily studied complex from Rhodobacter sphaeroides . 1 email m.r.jones@bristol.ac.uk 14 10 2008 © The Authors Journal compilation © 2009 Biochemical Society 2009 electron transfer membrane...

View article titled, The petite purple photosynthetic powerpack

Articles

Molecular architecture of the proton diode of cytochrome c oxidase

Peter Brzezinski, Joachim Reimann, Pia Ädelroth

Biochem Soc Trans (2008) 36 (6): 1169–1174.

DOI: https://doi.org/10.1042/BST0361169

Published: 19 November 2008

... Society 2008 electron transfer haem-copper oxidase membrane protein proton pump proton transfer redox reaction Cyt c O catalyses the oxidation of four molecules of cytochrome c and the reduction of O 2 to H 2 O ( eqn 1a ). The electrons are donated from the relatively positive ( P...

View article titled, Molecular architecture of the proton diode of cytochrome c oxidase

Articles

Electroneutral and electrogenic catalysis by dihaem-containing succinate:quinone oxidoreductases

C. Roy D. Lancaster, Elena Herzog, Hanno D. Juhnke, M. Gregor Madej, Florian G. Müller, Rajsekhar Paul, Philipp G. Schleidt

Biochem Soc Trans (2008) 36 (5): 996–1000.

DOI: https://doi.org/10.1042/BST0360996

Published: 19 September 2008

...C. Roy D. Lancaster; Elena Herzog; Hanno D. Juhnke; M. Gregor Madej; Florian G. Müller; Rajsekhar Paul; Philipp G. Schleidt Membrane protein complexes can support both the generation and utilization of a transmembrane electrochemical proton potential (Δ p ), either by supporting transmembrane...

View article titled, Electroneutral and electrogenic catalysis by dihaem-containing succinate:quinone oxidoreductases

Articles

Coarse-grained simulation: a high-throughput computational approach to membrane proteins

Mark S.P. Sansom, Kathryn A. Scott, Peter J. Bond

Biochem Soc Trans (2008) 36 (1): 27–32.

DOI: https://doi.org/10.1042/BST0360027

Published: 22 January 2008

...Mark S.P. Sansom; Kathryn A. Scott; Peter J. Bond An understanding of the interactions of membrane proteins with a lipid bilayer environment is central to relating their structure to their function and stability. A high-throughput approach to prediction of membrane protein interactions with a lipid...

View article titled, Coarse-grained simulation: a high-throughput computational approach to membrane proteins

Articles

The twin-arginine transport system: moving folded proteins across membranes

F. Sargent

Biochem Soc Trans (2007) 35 (5): 835–847.

DOI: https://doi.org/10.1042/BST0350835

Published: 25 October 2007

... has provided a wealth of biochemical, biophysical and genetic data. In general, three types of integral membrane protein have been identified as components of the Tat translocase ( Figure 2 ): TatA (also called Tha4 and Tha9 in the plant chloroplast system), TatB (Hcf106 in plants), and TatC...

View article titled, The twin-arginine transport system: moving folded proteins across membranes

Articles

The structure of the bacterial protein translocation complex SecYEG

I. Collinson

Biochem Soc Trans (2005) 33 (6): 1225–1230.

DOI: https://doi.org/10.1042/BST0331225

Published: 26 October 2005

... ]. membrane protein protein translocation secretion SecYEG translocon Polypeptide transport and import usually occurs when they are in an unfolded state. In rare cases, proteins that necessarily fold before their transport across a membrane are translocated by the so-called TAT (twin arginine...

View article titled, The structure of the bacterial protein translocation complex SecYEG

Includes: Multimedia, Supplementary data

Articles

Do protein–lipid interactions determine the recognition of transmembrane helices at the ER translocon?

S.H. White, G. von Heijne

Biochem Soc Trans (2005) 33 (5): 1012–1015.

DOI: https://doi.org/10.1042/BST0331012

Published: 26 October 2005

...S.H. White; G. von Heijne Membrane-protein integration, folding and assembly processes in vivo depend on complex targeting, translocation, chaperoning, and sorting machineries that somehow read the ‘molecular code’ built into the nascent polypeptide, ultimately producing a properly folded protein...

View article titled, Do protein–lipid interactions determine the recognition of transmembrane helices at the ER translocon?

Articles

Lipids in and around photosynthetic reaction centres

P.K. Fyfe, M.R. Jones

Biochem Soc Trans (2005) 33 (5): 924–930.

DOI: https://doi.org/10.1042/BST0330924

Published: 26 October 2005

... the binding of cofactors and the provision of structural support. The challenges of modelling surface-associated electron density features such as lipids, detergents, small amphiphiles and ions are discussed. © 2005 The Biochemical Society 2005 detergent lipid membrane protein protein engineering...

View article titled, Lipids in and around photosynthetic reaction centres

Articles

Crystallographic and single-particle analyses of native- and nucleotide-bound forms of the cystic fibrosis transmembrane conductance regulator (CFTR) protein

N.H. Awayn, M.F. Rosenberg, A.B. Kamis, L.A. Aleksandrov, J.R. Riordan, R.C. Ford

Biochem Soc Trans (2005) 33 (5): 996–999.

DOI: https://doi.org/10.1042/BST0330996

Published: 26 October 2005

...N.H. Awayn; M.F. Rosenberg; A.B. Kamis; L.A. Aleksandrov; J.R. Riordan; R.C. Ford Cystic fibrosis, one of the major human inherited diseases, is caused by defects in the CFTR (cystic fibrosis transmembrane conductance regulator), a cell-membrane protein. CFTR acts as a chloride channel which can...

View article titled, Crystallographic and single-particle analyses of native- and nucleotide-bound forms of the cystic fibrosis transmembrane conductance regulator (CFTR) protein

Articles

Specific protein–lipid interactions in membrane proteins

C. Hunte

Biochem Soc Trans (2005) 33 (5): 938–942.

DOI: https://doi.org/10.1042/BST0330938

Published: 26 October 2005

...C. Hunte Many membrane proteins selectively bind defined lipid species. This specificity has an impact on correct insertion, folding, structural integrity and full functionality of the protein. How are these different tasks achieved? Recent advances in structural research of membrane proteins...

View article titled, Specific protein–lipid interactions in membrane proteins

Articles

Simulation studies of the interactions between membrane proteins and detergents

P.J. Bond, J. Cuthbertson, M.S.P. Sansom

Biochem Soc Trans (2005) 33 (5): 910–912.

DOI: https://doi.org/10.1042/BST0330910

Published: 26 October 2005

...P.J. Bond; J. Cuthbertson; M.S.P. Sansom Interactions between membrane proteins and detergents are important in biophysical and structural studies and are also biologically relevant in the context of folding and transport. Despite a paucity of high-resolution data on protein–detergent interactions...

View article titled, Simulation studies of the interactions between membrane proteins and detergents

Articles

Rewiring photosynthesis: engineering wrong-way electron transfer in the purple bacterial reaction centre

M.C. Wakeham, M.R. Jones

Biochem Soc Trans (2005) 33 (4): 851–857.

DOI: https://doi.org/10.1042/BST0330851

Published: 01 August 2005

.... Spectroscopic evidence has been presented that suggests that both cofactor branches catalyse membrane-spanning electron transfer in Type I reaction centres, although this may not universally be the case (see [ 24 – 27 ] and references therein). bacteriochlorophyll electron transfer membrane protein...

View article titled, Rewiring photosynthesis: engineering wrong-way electron transfer in the purple bacterial reaction centre

Articles

High-level transcription of large gene regions: a novel T 7 RNA-polymerase-based system for expression of functional hydrogenases in the phototrophic bacterium Rhodobacter capsulatus

T. Drepper, S. Arvani, F. Rosenau, S. Wilhelm, K.-E. Jaeger

Biochem Soc Trans (2005) 33 (1): 56–58.

DOI: https://doi.org/10.1042/BST0330056

Published: 01 February 2005

... 2005 heterologous expression membrane protein redox enzyme Rhodobacter capsulatus T 7 RNA-polymerase uptake hydrogenase International Hydrogenases Conference 2004: Independent Meeting held at the University of Reading, 24–29 August 2004. Edited by R. Cammack (King's College London...

View article titled, High-level transcription of large gene regions: a novel T 7 RNA-polymerase-based system for expression of functional hydrogenases in the phototrophic bacterium Rhodobacter capsulatus

Articles

Annexin A2 expression during cellular differentiation in myeloid cell lines

W.S. Gilmore, S. Olwill, H. McGlynn, H.D. Alexander

Biochem Soc Trans (2004) 32 (6): 1122–1123.

DOI: https://doi.org/10.1042/BST0321122

Published: 26 October 2004

... (email WS.Gilmore@ulster.ac.uk ). 8 7 2004 © 2004 The Biochemical Society 2004 annexin differentiation haematopoiesis membrane protein Annexins are a family of homologous calcium-dependent proteins, named because of their ability to annex anionic phospholipid membranes [ 1...

View article titled, Annexin A2 expression during cellular differentiation in myeloid cell lines

Articles

Immunoglobulin A1 proteases: a structure–function update

H.K. Parsons, S. Vitovski, J.R. Sayers

Biochem Soc Trans (2004) 32 (6): 1130–1132.

DOI: https://doi.org/10.1042/BST0321130

Published: 26 October 2004

... 1 To whom correspondence should be addressed (email J.R.Sayers@sheffield.ac.uk ). 27 7 2004 host–pathogen interaction immunoglobulin A1 (IgA1) membrane protein protease translocation type V secretion Neisseria meningitidis and Haemophilus influenzae are responsible...

View article titled, Immunoglobulin A1 proteases: a structure–function update

Articles

Tail-anchored protein biosynthesis at the endoplasmic reticulum: the same but different

S. High, B.M. Abell

Biochem Soc Trans (2004) 32 (5): 659–662.

DOI: https://doi.org/10.1042/BST0320659

Published: 26 October 2004

...S. High; B.M. Abell The post-translational integration of tail-anchored proteins at the endoplasmic reticulum represents a novel and distinct pathway for membrane protein synthesis. Studies of various precursors, exemplified by the synaptobrevins and cytochrome b5 , indicate that multiple routes...

View article titled, Tail-anchored protein biosynthesis at the endoplasmic reticulum: the same but different

Articles

X-ray crystallographic studies for ligand–protein interaction changes in rhodopsin

T. Okada

Biochem Soc Trans (2004) 32 (5): 738–741.

DOI: https://doi.org/10.1042/BST0320738

Published: 26 October 2004

...: Biological Information Research Center, National Institute of Advanced Industrial Science and Technology, 2-41-6 Aomi, Koto-ku, Tokyo 135-0064, Japan (email t-okada@aist.go.jp ). 17 6 2004 © 2004 The Biochemical Society 2004 crystal structure G-protein-coupled receptor membrane protein...

View article titled, X-ray crystallographic studies for ligand–protein interaction changes in rhodopsin

Articles

The use of isotope-coded affinity tags (ICAT) to study organelle proteomes in Arabidopsis thaliana

T.P.J. Dunkley, P. Dupree, R.B. Watson, K.S. Lilley

Biochem Soc Trans (2004) 32 (3): 520–523.

DOI: https://doi.org/10.1042/bst0320520

Published: 01 June 2004

...: a Biochemical Society Focused Meeting held at Rothamsted Research, Harpenden, 25 November 2003 25 November 2003 © 2004 Biochemical Society 2004 Golgi isotope-coded affinity tag (ICAT) membrane protein organelle quantitative proteomics Abbreviations used: 2D, two-dimensional...

View article titled, The use of isotope-coded affinity tags (ICAT) to study organelle proteomes in Arabidopsis thaliana

Articles

Mercury transport and resistance

N. L. Brown, Y.-C. Shih, C. Leang, K. J. Glendinning, J. L. Hobman, J. R. Wilson

Biochem Soc Trans (2002) 30 (4): 715–718.

DOI: https://doi.org/10.1042/bst0300715

Published: 01 August 2002

... © 2002 Biochemical Society 2002 heavy metal ion transport membrane protein mercuric ion resistance Biometals 2002: Third International Biometals Symposium: Focused Meeting Organized by S. Andrews (School of Animal and Microbial Sciences, University of Reading), R. Cammack, R. W...

View article titled, Mercury transport and resistance

Articles

Impact of oligomerization on the function of the human serotonin transporter

J. A. Schmid, H. Just, H. H. Sitte

Biochem Soc Trans (2001) 29 (6): 732–736.

DOI: https://doi.org/10.1042/bst0290732

Published: 01 November 2001

...J. A. Schmid; H. Just; H. H. Sitte The formation of oligomeric structures has been proposed for a large number of membrane proteins, including G-protein-coupled receptors and ion channels. Biochemical studies employing gel filtration, cross-linking or co-immunoprecipitation techniques showed...

View article titled, Impact of oligomerization on the function of the human serotonin transporter

Articles

Structural model of a voltage-gated potassium channel based on spectroscopic data

P. I. Haris

Biochem Soc Trans (2001) 29 (4): 589–593.

DOI: https://doi.org/10.1042/bst0290589

Published: 01 August 2001

...P. I. Haris It is estimated that membrane proteins comprise as much as 30% of most genomes. Yet our knowledge of membrane-protein folding is still in its infancy. Consequently, there is a great need for developing approaches that can further advance our understanding of how peptides and proteins...

View article titled, Structural model of a voltage-gated potassium channel based on spectroscopic data

Articles

De novo design, synthesis and characterization of membrane-active peptides

J. D. Lear, H. Gratkowski, W. F. DeGrado

Biochem Soc Trans (2001) 29 (4): 559–564.

DOI: https://doi.org/10.1042/bst0290559

Published: 01 August 2001

...J. D. Lear; H. Gratkowski; W. F. DeGrado Our current level of understanding of membrane-protein folding is primitive, but it is beginning to advance. Previously [Choma, Gratkowski, Lear and DeGrado (2000) Nat. Struct. Biol. 7, 161–166], we described studies of the association in detergent micelles...

View article titled, De novo design, synthesis and characterization of membrane-active peptides

NARROW

Filters

The energetics of protein–lipid interactions as viewed by molecular simulations

Crystallizing the function of the magnetosome membrane mineralization protein Mms6

Preparation of purified GPCRs for structural studies

The quest to achieve the detailed structural and functional characterization of CymA

Membrane proteins: from bench to bits

Lipid–protein interactions

Surfactant-free purification of membrane proteins with intact native membrane environment

Unravelling the folding and stability of an ABC (ATP-binding cassette) transporter

Lipid–protein interactions as determinants of membrane protein structure and function

Endogenous ion channel complexes: the NMDA receptor

Membrane proteins in nanotechnology

The bacterial respiratory nitric oxide reductase

The petite purple photosynthetic powerpack

Molecular architecture of the proton diode of cytochrome c oxidase

Electroneutral and electrogenic catalysis by dihaem-containing succinate:quinone oxidoreductases

Coarse-grained simulation: a high-throughput computational approach to membrane proteins

The twin-arginine transport system: moving folded proteins across membranes

The structure of the bacterial protein translocation complex SecYEG

Do protein–lipid interactions determine the recognition of transmembrane helices at the ER translocon?

Lipids in and around photosynthetic reaction centres

Crystallographic and single-particle analyses of native- and nucleotide-bound forms of the cystic fibrosis transmembrane conductance regulator (CFTR) protein

Specific protein–lipid interactions in membrane proteins

Simulation studies of the interactions between membrane proteins and detergents

Rewiring photosynthesis: engineering wrong-way electron transfer in the purple bacterial reaction centre

High-level transcription of large gene regions: a novel T 7 RNA-polymerase-based system for expression of functional hydrogenases in the phototrophic bacterium Rhodobacter capsulatus

Annexin A2 expression during cellular differentiation in myeloid cell lines

Immunoglobulin A1 proteases: a structure–function update

Tail-anchored protein biosynthesis at the endoplasmic reticulum: the same but different

X-ray crystallographic studies for ligand–protein interaction changes in rhodopsin

The use of isotope-coded affinity tags (ICAT) to study organelle proteomes in Arabidopsis thaliana

Mercury transport and resistance

Impact of oligomerization on the function of the human serotonin transporter

Structural model of a voltage-gated potassium channel based on spectroscopic data

De novo design, synthesis and characterization of membrane-active peptides

Email alerts

CONNECT

EXPLORE

Email alerts

CONNECT

EXPLORE

This Feature Is Available To Subscribers Only