... by Portland Press Limited on behalf of the Biochemical Society 2020 DNA polymerases DNA replication and recommbination mutagenesis PCNA proten-protein interaction translesion DNA synthesis DNA replication is one of the most basic cellular processes that ensures accurate duplication...

... is associated with an increase in mutagenesis. The DNA repair system can become ineffective or saturated in such conditions [ 80 , 94–96 ]. Cell cycle timing, population density, and nucleoid compaction also appear to modulate mutation rates in bacteria [ 16 , 93 , 97 , 98 ]. Imaging of DNA mismatches enables...

... 2017 © 2017 The Author(s) 2017 This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . cowpea mosaic virus cryo-electron microscopy mutagenesis transient...

... side of the active site to the canopy. The mechanism of hydrogen activation has been dissected by site-directed mutagenesis to identify the catalytic base responsible for splitting molecular hydrogen and possible proton transfer pathways to/from the active site. Previous reported attempts to mutate...

... future opportunities to better understand GT domain–domain interactions. carbohydrate directed evolution enzymatic synthesis glycosyltransferase mutagenesis protein engineering A growing appreciation for the important biological roles and the therapeutic potential of carbohydrates...

... The Authors Journal compilation © 2013 Biochemical Society 2013 alanine scan family A GPCRs family B GPCRs mutagenesis secretin-like GPCR structure–activity relationship CGRP is an abundant 37-amino-acid neuropeptide that is especially found in the sensory nervous system. CGRP is released...

... [ 35 ] but is less soluble at low pH [ 36 ]. In order to explore this possibility for change throughout the nisin molecule, a variety of PCR-based approaches have been used, including site-directed, random and saturation mutagenesis have been used. In the first instance, this fundamental analysis...

... calcitonin mutagenesis receptor activity-modifying protein secretin-like G-protein-coupled receptor structure–activity relationship GPCRs form a number of families. The best characterized of these are the rhodopsin-like or family A GPCRs. The secretin-like or family B GPCRs form receptors...

...). Previous studies have used a chimaeric and site-specific mutagenesis approach to identify three residues in the putative tenth transmembrane helix of SV2A that, when mutated, alter binding of LEV and related racetam derivatives to SV2A. In the present paper, we report a combined modelling and mutagenesis...

..., and specific interactions between GABA and some of these residues have been determined. In the present paper, I show how a cation–π interaction for one of the binding site residues has allowed the production of models of GABA docked into the binding site, and these orientations are supported by mutagenesis...

... both gene-driven (reverse genetics) and phenotype-driven (forward genetics) approaches. A high-efficiency ENU approach results in approx. 25 functional mutations per genome; most of these will result in hypomorphic alleles. Our group has recently begun using ENU mutagenesis as a tool for understanding...

... perturbations. Random mutagenesis was induced, and individual mutagenized chromosomes were recovered by classic crossing schemes involving phenotypical markers and balancer chromosomes. In wild-type larvae, ferritin is predominantly localized in the brain, in regions of the intestine, in wreath cells...

... mutagenesis translesion synthesis ubiquitin The stability of our genetic material is endangered both by DNA-damaging agents from the environment and by endogenous problems during DNA replication arising from spontaneous damage [ 1 ]. DNA repair pathways, the cell's first line of defence against many...

... for secretin binding based on receptor mutagenesis and photoaffinity labelling studies. Full agonist analogues of secretin incorporating a photolabile moiety at various positions throughout the pharmacophore covalently label residues within this region, while only N-terminal probes have labelled the core...

... by an inhibition, mutagenesis and homology-modelling approach. Crystal structure and homology modelling indicate that both enzymes possess a narrow constriction that separates an entrance lipophilic channel from the active-site cavity. The role of the constriction as a mobile gate that permits substrate passage...

... of their antifungal activity. All experiments described here were performed on S. cerevisiae strain W303-1B (genotype MAT α ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 can 1-100 ) and mutants derived from this strain by EMS (ethane methyl sulphonate) mutagenesis. Susceptibility testing was performed by drop...

.... Kobilka B.K. J. Biol. Chem. 2001 276 24433 24436 cAMP regulatory element (CRE)-luciferase glucagon G-protein-coupled receptor (GPCR) mutagenesis receptor activation ternary complex The extended ternary complex model for GPCR (G-protein-coupled receptor) activation suggests...

..., methylglyoxal, mutagenesis, multidrug resistance, tumour. Abbreviations used: AGE, advanced glycation end-product; dG-G, 3-(2 -deoxyribosyl)- 6,7-dihydro-6,7-dihydroxyimidazo[2,3-b]purin-9(8)one; dG-MG, 6,7-dihydro-6,7-dihydroxy-6- methylimidazo-[2,3-b]purine-9(8)one; CMdG, N2-carboxymethyldeoxyguanosine; gdC...

... in 2D and 3D, a Biochemical Society Focused Meeting held at University of Nottingham, 19–20 June 2003 20 June 2003 © 2003 Biochemical Society 2003 fluorescence spectroscopy mutagenesis secondary-structure prediction steroid receptor transactivation Abbreviations used...

..., irreversible conformational changes to convert them into the form capable of inserting into the membrane (Scheme 1) [7-lo]. Membrane- Key words: conformational change, metastable, model mem- branes, mutagenesis, receptor binding. Abbreviations used: APN, aminopeptidase N: Bt. Bacillus thuringiensis. 'To whom...