... be phosphorylated and, among these, histidine, aspartate and cysteine have well established roles in bacterial signalling pathways. The first of these, phosphohistidine, is the most unusual in that it is labile under many conditions used to study proteins in vitro and can exist as two different isomers...

... in higher eukaryotes. 1 email [email protected] 7 3 2013 © The Authors Journal compilation © 2013 Biochemical Society 2013 histidine kinase phosphohistidine phosphohistidine phosphatase response regulator Protein histidine phosphorylation was discovered...

... in signalling in eukaryotes, plants and fungi, roles for phosphohistidine are poorly defined in higher eukaryotes. Characterization of histidine phosphorylation aimed at elucidating such information is problematic due to the acid-labile nature of the phosphoramidate bond, essential for many of its biological...

...-phosphohistidine, whereas that from Walker-256 carcinosarcoma cells forms 3-phosphohistidine [ 13 ]. HHK from P. polycephalum only phosphorylates His 75 to form 1-phosphohistidine [ 7 ]. The HHK from S. cerevisiae has been purified to homogeneity [ 8 ] and partially characterized. The enzyme is a monomeric 32...