...Benjamin J. Hardy; Paul Curnow The major energy-producing reactions of biochemistry occur at biological membranes. Computational protein design now provides the opportunity to elucidate the underlying principles of these processes and to construct bioenergetic pathways on our own terms. Here, we...

...Paul Curnow The construction of artificial membrane proteins from first principles is of fundamental interest and holds considerable promise for new biotechnologies. This review considers the potential advantages of adopting a strictly minimalist approach to the process of membrane protein design...

... engineering becomes replaced by rational/computational approaches. This minireview will discuss some of the approaches to protein engineering of remote regions that have been developed in recent years and highlight their importance in the context of a more comprehensive approach to protein design...

... Fletcher J.M. Boyle A.L. Bruning M. Bartlett G.J. Vincent T.L. Zaccai N.R. Armstrong C.T. Bromley E.H.C. Booth P.J. Brady L. A basis set of de novo coiled-coil peptide oligomers for rational protein design and synthetic biology ACS Synth. Biol. 2012 1 240 250...

... with important practical applications. 1 To whom correspondence should be addressed (email [email protected] ) . 30 4 2015 © 2015 Authors; published by Portland Press Limited 2015 affinity reagent antibody protein design protein–protein interactions protein purification repeat...

...Pamela J.E. Rowling; Elin M. Sivertsson; Albert Perez-Riba; Ewan R.G. Main; Laura S. Itzhaki Studying protein folding and protein design in globular proteins presents significant challenges because of the two related features, topological complexity and co-operativity. In contrast, tandem-repeat...

... compartments. 1 These authors contributed equally to this review. 2 To whom correspondence should be addressed (email [email protected] ) . 15 4 2015 © 2015 Authors; published by Portland Press Limited 2015 αRep combinatorial library crystal structure protein...

... molecular recognition protein design repeat proteins In parallel, the ever versatile DARPin framework was recently used for selective and specific DNA binding. Scholz et al. [ 38 ] described the redesign of DARPins for selective recognition of human telomere G-quadruplex DNA with nanomolar affinity...

... [email protected] ) . 20 4 2015 © 2015 Authors; published by Portland Press Limited 2015 biomolecular templating hybrid materials protein design repeat proteins tetratricopeptide repeat nanostructures Nowadays, the industrial sector demands complicated and sophisticated...

... developed a computational saturated mutagenesis protocol [ 11 ] based on the framework of the ORBIT protein design package [ 12 ]. The protocol utilizes an energy function that has been specifically optimized by our group for design of PPIs [ 13 , 14 ]. For a given PPI with an available high-resolution...

... decade, protein engineering still faces numerous challenges. Often, designs do not work as anticipated and they still require substantial redesign. The present review focuses on the successes, the challenges and the limitations of rational protein design today. 1 These authors contributed...

... 101 14102 14107 38 Grove T.Z. Osuji C.O. Forster J.D. Dufresne E.R. Regan L. Stimuli-responsive smart gels realized via modular protein design J. Am. Chem. Soc. 2010 132 14024 14026 39 Grove T.Z. Regan L. Cortajarena A.L. Nanostructured...

... that those same residues placed appropriately will definitely yield the same function in some other protein structural family. © The Authors Journal compilation © 2012 Biochemical Society 2012 electron transfer maquette oxidoreductase protein design protein engineering synthetic protein...

... leucine-zipper nanostructured material peptide α-helix protein design self-assembling peptide Figure 1 A dimeric coiled-coil superhelix as a building block for synthetic nanostructures ( A ) The block is shown as a local region of a natural assemblage – the muscle protein tropomyosin. ( B...