... with the translocation of protons across the membrane. Electron microscopy revealed the two-part structure of the complex consisting of a peripheral and a membrane arm. The peripheral arm contains all known cofactors and the NADH-binding site, whereas the membrane arm has to be involved in proton translocation. Owing...

... to the solid surface [ 18 ]. Such a control of the orientation of membrane protein is crucial when the vectorial function of the protein is addressed [ 17 ]. gold surface ion transfer membrane potential proton translocation surface-enhanced IR absorption spectroscopy (SEIRAS) vibrational spectroscopy...

... was varied by the addition of 50 mM Mes/NaOH, 100 mM NaCl and 5 mM MgCl 2 (pH 6.0). The assay ( B ) contained 30 μg of complex I in 50 mM Mes/NaOH, 100 mM NaCl and 5 mM MgCl 2 (pH 6.0). complex I Escherichia coli NADH dehydrogenase NADH:ubiquinone oxidoreductase proton translocation sodium...