... in the pursuit of high-resolution structural data for several membrane proteins, and we have applied some of the princples developed in previous studies to the expression and purification of CFTR. We have overexpressed this protein, along with evolutionarily diverse orthologues, in Saccharomyces cerevisiae...

...), most of which are cysteine proteases. This, taken together with the rapid proteasomal degradation of some species of ubiquitinated proteins, results in difficulties in detecting modified targets. In this review, practical approaches developed for the detection, purification and characterization...

... compilation © 2007 Biochemical Society 2007 catalytic scavenger functional state human phosphate-binding protein paraoxonase protein stability purification HDL offers a hydrophobic harbour for PON1 after the secretion by liver and provides a stabilizing environment for PON1 function(s) [ 13...

... was increased, indicating that, at higher pH, there is an increase in the high-spin state of the protein. cytochrome c ′ Neisseria meningitidis nitrosyl complex nitric oxide purification spectroscopy Cytochrome c ′, a cytochrome with a C-terminal, covalently attached haem and unusual...

... correspondence should be addressed (e-mail [email protected] ) 26 6 2000 © 2000 Biochemical Society 2000 defence oxylipins purification LOX, lipoxygenase Pmg, Phytophtora sojae crude cell-wall elicitor protein Lipids and Signalling: Oxylipins I Lipoxygenase...