...-time, single-molecule dynamics crucial for understanding RNA function. This review explores the transformative potential of single-molecule force spectroscopy using optical tweezers, showcasing its capability to directly probe time-dependent structural rearrangements of individual RNA molecules...

... the technical developments that now enable mRNA transcripts and their translation to be visualized at single-molecule resolution in both fixed and live cells. These tools include single-molecule fluorescence in situ hybridization (smFISH) to visualize mRNA in fixed cells, MS2/PP7 labelling for live mRNA imaging...

... and distributed under the Creative Commons Attribution License 4.0 (CC BY) . chromosomes condensin DNA binding single-molecule SMC structural biology DNA binds to Ycs4 in the condensin bridged state. The hinge could additionally contribute to DNA binding. DNA binding promotes closure...

... techniques based on single-molecule detection that have helped overcome this challenge. These studies focus on the assembly dynamics and splicing kinetics in real-time, which help understanding of spliceosomal assembly and catalysis. Bringing together the BS and the SS must require extensive structural...