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Keywords: solubilization
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Articles
Overcoming bottlenecks in the membrane protein structural biology pipeline
Available to Purchase
Journal:
Biochemical Society Transactions
Biochem Soc Trans (2016) 44 (3): 838–844.
Published: 09 June 2016
... of not working with the sequence of interest. Novel detergents such as glucose neopentyl glycol (GNG), maltose neopentyl glycol (MNG) and calixarene-based detergents can improve protein stability without compromising their solubilizing properties. Styrene maleic acid lipid particles (SMALPs) focus on retaining...
Articles
Surfactant-free purification of membrane proteins with intact native membrane environment
Available to PurchaseMohammed Jamshad, Yu-Pin Lin, Timothy J. Knowles, Rosemary A. Parslow, Craig Harris, Mark Wheatley, David R. Poyner, Roslyn M. Bill, Owen R.T. Thomas, Michael Overduin, Tim R. Dafforn
Journal:
Biochemical Society Transactions
Biochem Soc Trans (2011) 39 (3): 813–818.
Published: 20 May 2011
.... These approaches move away from solubilization methods based on the use of small surfactants, which have been shown to suffer from significant practical problems. Instead, the present review focuses on methods that stem from the field of nanotechnology and use a range of reagents that fragment the membrane...