...Mika Jormakka Iron is a vital trace element for almost all organisms, and maintaining iron homeostasis is critical for human health. In mammals, the only known gatekeeper between intestinally absorbed iron and circulatory blood plasma is the membrane transporter ferroportin (Fpn...

...Shamayeeta Ray; Rachelle Gaudet A repertoire of transporters plays a crucial role in maintaining homeostasis of biologically essential transition metals, manganese, and iron, thus ensuring cell viability. Elucidating the structure and function of many of these transporters has provided substantial...

...; by examining the ligand-binding domain of the regulator and the transporter, we propose that nucleotides are the likely substrate for an enigmatic uncharacterized BMC of unknown function. Correspondence: Cheryl A. Kerfeld ( [email protected] ) 13 4 2021 2 6 2021 4 6 2021 © 2021...

.... hydrogen-deuterium exchange mass spectrometry integrative modelling membrane proteins transport Correspondence: Argyris Politis ( [email protected] ) 3 4 2020 2 6 2020 4 6 2020 Figure 1. Methods of comparison between simulated and experimental HDX...

...Alisa A. Garaeva; Dirk J. Slotboom Membrane transporters are integral membrane proteins that mediate the passage of solutes across lipid bilayers. These proteins undergo conformational transitions between outward- and inward-facing states, which lead to alternating access of the substrate-binding...

... knowledge of the roles of biological membranes can be exploited to enhance the productivity of cell factories. Improving existing, and developing new, cell factories requires a deep understanding of the mechanisms by which substances are transported into, within, and out of the cells. Embedding...

... in these processes is the transport of signalling molecules from the cytoplasm to the nucleus. The SRF (serum-response factor) and YAP (Yes-associated protein)/TAZ (transcriptional co-activator with PDZ-binding motif) pathways are known mediators of this process in multiple cell types, including mesenchymal stem...

... our current knowledge of the molecular mechanisms involved in the handling of iron by astrocytes. Cultured astrocytes efficiently take up iron as ferrous or ferric iron ions or as haem by specific iron transport proteins in their cell membrane. In addition, astrocytes accumulate large amounts of iron...

... dendrite neuron organelle plasticity transport A major challenge for all cells is to traffic integral membrane proteins, secreted factors and lipids to appropriate subcellular domains in the right amount at the right time. With surface areas up to 10000-fold greater than typical mammalian cells...

... incorporated to these levels, many of these analogues induce the synthesis of a regulatory protein, antizyme, which inhibits both polyamine synthesis and the transporter they used to enter the cell. Thus this feedback system allows steady-state maintenance of effective cellular doses of such analogues...

... with that of agmatine and they are thus able to inhibit agmatine transport in liver mitochondria. The presence of the guanidine group is essential for an optimal effect, since AO-Agm and NGPG display competitive inhibition, whereas that of GAPA is non-competitive. NGPG is the most effective inhibitor ( K i =0.86 mM...

...D.R. Voelker Phosphatidylserine (PtdSer) is synthesized in the endoplasmic reticulum and its subdomains associated with the mitochondria [MAM (mitochondria-associated membrane)] and subsequently transported to the loci of the PtdSer decarboxylases, Pds1p (phosphatidylserine decarboxylase 1 encoded...

... carriers transport For the past 30 years, our laboratory has been interested in various aspects of transmembrane molecular transport [ 1 , 2 ]. For the purpose of understanding these processes, we have applied both bioinformatic and molecular biological approaches [ 3 , 4 ]. As part of our...

.... It indicates a process which is described as bipartite gating and allows for a rational distinction between the binding event and the transport process. Biochemical Society Transactions (2002) Volume 30, part 4 Bipartite gating in the outer membrane protein FecA D. van der Helm R. Chakraborty*f, A. D...

...D. Hubmacher; B. F. Matzanke; S. Anemüller The iron transport in the extremely halophilic Euryarchaeon Halobacterium salinarum JW5 was investigated. Experiments to detect endogenous siderophores from H. salinarum failed, but it was able to utilize exogenous siderophores. Measurement of the uptake...

... prior to uptake by members of the Fre family of plasma-membrane metalloreductases. Following reduction and release from the siderophore, the iron is then taken up via the high-affinity ferrous transport system. A set of transporters that specifically recognizes siderophore-iron chelates is also...

... chaperones in A. thaliana based on the information available from the complete sequence of its genome. 1 To whom correspondence should be addressed (e-mail [email protected] ) 13 3 2002 © 2002 Biochemical Society 2002 Arabidopsis homoeostasis transport...

...S. Apparsundaram; S. M. Ferguson; R. D. Blakely In cholinergic neurons, a specific requirement for precursor choline in the biosynthesis of acetylcholine (ACh) is thought to be sustained by a presynaptic, hemicholinium-3 (HC-3)-sensitive choline transporter (CHT). This transporter exhibits...

... to facilitate purification. Growth conditions were optimized in 1–25-litre cultures of E. coli host strains to amplify the expression of each protein; the retention of activity was confirmed by assays of antibiotic resistance in vivo and/or assays of energized transport activity in vitro with synthetic...