...Takuma Tsuji; Toyoshi Fujimoto The membrane raft has been a focus of intensive research for the past two decades. Liquid-ordered domains form in artificial liposomes containing sterol and saturated lipids, but their presence in living cell membranes has been controversial. The yeast vacuole...

...Carine de Marcos Lousa; Jurgen Denecke Soluble hydrolases represent the main proteins of lysosomes and vacuoles and are essential to sustain the lytic properties of these organelles typical for the eukaryotic organisms. The sorting of these proteins from ER residents and secreted proteins...

... these compartments, though the precise mechanisms are still enigmatic. Among the cellular organelles, the lysosome/vacuole stands out as a central hub, where multiple pathways merge. Importantly, the delivered material is degraded and the monomers are recycled for further usage, which explains its wide variety...

... vacuoles, membrane-bound acidic organelles, which share many similarities to plant vacuoles and mammalian lysosomes, are also involved in the regulation of apoptosis and that the vacuolar protease Pep4p, orthologue of the human CatD, is released from the vacuole into the cytosol in response to acetic acid...

... three adjacent z -stacks. In an effort to focus on the distribution of vacuoles labelled by TIPs in a model plant species, and to reduce the risk of localization artefacts due to overexpression, we mapped the expression of the three isoforms which were initially used to discriminate between LVs...

..., the endocytic tracers like ssGFP are not degraded in lysosomes. Instead, they accumulate in large vacuoles that are positive for both late endosomal and lysosomal markers. CUP-5 is the C. elegans functional orthologue of human mucolipin-1 [ 8 ]. Mutations in mucolipin-1 cause MLIV (mucolipidosis type IV...