Monoclonal antibodies to human α1-ietoprotein (AFP) have been compared with a conventionally produced antiserum using radioimmunoassay and two-site immunoradiometric techniques. A low-affinity antibody, which proved inadequate for use in a radioimmunoassay, gave asatisfactory dose-response Curve in a rapid two-site assay. A higher-affinity antibody yielded a simple, rapid, and sensitive two-site assay suitable for routine measurement of serum AFP.
Research Article| October 01 1981
Two-site assay of human α1-fetoprotein using125I-labelled monoclonal antibodies
Heather A. Kemp;
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Ian Weeks, Heather A. Kemp, J. Stuart Woodhead; Two-site assay of human α1-fetoprotein using125I-labelled monoclonal antibodies. Biosci Rep 1 October 1981; 1 (10): 785–791. doi: https://doi.org/10.1007/BF01114801
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