The metabolism of radioactively labelled D-glucose, L-glutamine, and L-glutamate has been determined in a glycolytic mutant of Chinese-hamster ovary cells, R1.1.7, and in its parent, CHO-K1. The complete oxidation of glucose via the TCA-cycle is negligible in both cell types, but there is significant oxidation of carbon-1. CHO-K1 cells derive most of their energy from glycolysis and are independent of respiration in the short term. R1.1.7 cells are respiration-dependent and are rapidly killed by respiratory inhibitors. Both cell types oxidize L-glutamine and L-glutamate, but the oxidation of these substrates does not appear sufficient to satisfy completely the energy requirements of R1.1.7 cells.
Regulation of carbohydrate metabolism in cultured mammalian cells: Energy provision in a glycolytic mutant
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Michael J. Morgan, Kathleen M. Bowness, Pelin Faik; Regulation of carbohydrate metabolism in cultured mammalian cells: Energy provision in a glycolytic mutant. Biosci Rep 1 October 1981; 1 (10): 811–817. doi: https://doi.org/10.1007/BF01114804
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