The use of antiserum with specific reactivity toward fat-cell surface antigen(s) to follow the progression of 3T3-L₁ preadipocyte differentiation in vitro

Using an i n d i r e c t, labelled-second-antibody cellular immunoassay technique, an adipocyte-specific antiserum haS been investigated. Components of the antiserum were shown to bind to differentiated 3T3-L₁ cells; the cellular capacity for binding increased progressively during the induced differentiation of these ceils in vitro.