Escherichia coli malate dehydrogenase has been isolated in homogeneous form by a procedure employing chromatography on DEAE-cellulose, 5′-AMP-Sepharose, and Sephacryl-200. It is composed of two identical polypeptide chains each of Mr = 32 500. Like porcine mitochondrial malate dehydrogenase, it is devoid of tryptophan, but otherwise it is not particularly more similar in composition to one of the eukaryotic isozymes than to the other. However, amino-terminal sequence analysis of the first 36 residues shows remarkable similarity of the bacterial and mitochondrial enzymes (69% identical residues) in contrast to the cytoplasmic form (27%). The two porcine heart enzymes are identical in 24t% of the positions compared. These results clearly establish that all three forms of malate dehydrogenase have evolved from a common precursor and that the prokaryotic and mitochondrial forms have retained sequences that are much closer to the ancestral one than the cytoplasmic enzyme. These findings appear to further substantiate the endosymbiotic hypothesis for the origin of the mitochondrion.
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June 01 1981
Malate dehydrogenase: Isolation from E. coli and comparison with the eukaryotic mitochondrial and cytoplasmic forms
Ross T. Fernley;
Ross T. Fernley
1Department of Biological Chemistry, Washington University School of Medicine, St. Louis, MO 63110, U.S.A.
*Howard Florey Institute for Experimental Physiology and Medicine, University of Melbourne, Parkville, Victoria 3052, Australia
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Steven R. Lentz;
Steven R. Lentz
1Department of Biological Chemistry, Washington University School of Medicine, St. Louis, MO 63110, U.S.A.
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Ralph A. Aradshaw
Ralph A. Aradshaw
1Department of Biological Chemistry, Washington University School of Medicine, St. Louis, MO 63110, U.S.A.
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Publisher: Portland Press Ltd
Received:
May 18 1981
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1981 The Biochemical Society
1981
Biosci Rep (1981) 1 (6): 497–507.
Article history
Received:
May 18 1981
Citation
Ross T. Fernley, Steven R. Lentz, Ralph A. Aradshaw; Malate dehydrogenase: Isolation from E. coli and comparison with the eukaryotic mitochondrial and cytoplasmic forms. Biosci Rep 1 June 1981; 1 (6): 497–507. doi: https://doi.org/10.1007/BF01121583
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