For the development of immunological contraception, attention is being concentrated on the possibility of using a sperm membrane antigen. Boar sperm membrane was extracted with triton-X 100 and fractionated by Sephadex G-150 column chromatography. The glycosylated and nonglycosylated portions of protein peaks from the gel filtration were obtained by fractionating on concanavalin A-Sepharose and eluting the bound protein with 0.3 M methyl mannoside. A glycosylated fraction was found to induce sperm agglutinating antibodies in rabbit. The partially purified protein has a molecular weight of 30 kilodaltons, as determined by sodium dodecyl polyaccyrlamide gel electrophoresis. Further work is planned on the histochemical determination of the origin of this protein and species cross-activity of the antibody.
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April 01 1990
Partial purification of a boar sperm membrane protein inducing sperm agglutinating antibody Available to Purchase
Oyewole Adeyemo;
Oyewole Adeyemo
1Department of Veterinary Anatomy, University of Ibadan, Ibadan, Nigeria
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E. O. Okegbile;
E. O. Okegbile
2Department of Biochemistry, University of Ibadan, Ibadan, Nigeria
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O. O. Olorunsogo
O. O. Olorunsogo
2Department of Biochemistry, University of Ibadan, Ibadan, Nigeria
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Publisher: Portland Press Ltd
Received:
May 24 1989
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1990 Plenum Publishing Corporation
1990
Biosci Rep (1990) 10 (2): 131–139.
Article history
Received:
May 24 1989
Citation
Oyewole Adeyemo, E. O. Okegbile, O. O. Olorunsogo; Partial purification of a boar sperm membrane protein inducing sperm agglutinating antibody. Biosci Rep 1 April 1990; 10 (2): 131–139. doi: https://doi.org/10.1007/BF01116572
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