The cooperative cell kinetic actions of ET-1 with TGF-α or EGF in normal rat kidney fibroblasts (NRK-49F) and KNRK cells (Kirsten MSV transformed) were analyzed by [3H]-thymidine incorporation assay and flow cytometry. A marked synergistic effect of TGF-α and ET-1 (or EGF and ET-1) on DNA synthesis and G1 to S transition was observed in NRK cells; 15–20% S for TGF-α and 12% S for ET-1 alone but 45–50% S in combination. There was no detectable effect on cell cycle kinetics by TGF-α (1 ng/ml) or EGF (1 ng/ml) plus ET-1 (1 ng/ml) in KNRK cells treated for 22 hours. Insulin, insulin-like growth factor I (IGF-I), fibroblast growth factor (FGF), platelet derived growth factor (PDGF), and transforming growth factor β (TGF-β) were also tested and found to have no significant synergistic effects on ET-1 actions. Our findings suggest that the combination of TGF-α (EGF) and ET-1 is an important part of an intricate network which coordinates progression of G1 to S phase in normal cells.
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June 01 1991
Synergistic effects of endothelin-1 (ET-1) and transforming growth factor alpha (TGF-α) or epidermal growth factor (EGF) on DNA replication and G1 to S phase transition
Yun-Chi Yeh;
Yun-Chi Yeh
1Department of Biochemistry and Molecular Biology, College of Medicine, University of Arkansas for Medical Sciences, Little Rock, AR 72205
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E. Robert Burns;
E. Robert Burns
2Department of Anatomy, College of Medicine, University of Arkansas for Medical Sciences, Little Rock, AR 72205
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John Yeh;
John Yeh
3Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115
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Hsing-Wu Yeh
Hsing-Wu Yeh
1Department of Biochemistry and Molecular Biology, College of Medicine, University of Arkansas for Medical Sciences, Little Rock, AR 72205
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Publisher: Portland Press Ltd
Received:
March 23 1991
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1991 Plenum Publishing Corporation
1991
Biosci Rep (1991) 11 (3): 171–180.
Article history
Received:
March 23 1991
Citation
Yun-Chi Yeh, E. Robert Burns, John Yeh, Hsing-Wu Yeh; Synergistic effects of endothelin-1 (ET-1) and transforming growth factor alpha (TGF-α) or epidermal growth factor (EGF) on DNA replication and G1 to S phase transition. Biosci Rep 1 June 1991; 11 (3): 171–180. doi: https://doi.org/10.1007/BF01182486
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