Erythrocytes from growth hormone-deficient children (GHd-children) (n=10) showed a statistically significant increase in insulin binding at low unlabeled insulin concentrations, together with a threefold decrease in apparent receptor affinity, as compared to control children (C) (n=11). Scatchard analysis of the binding data using the two-site model revealed that both the receptor concentration R1 [GHd-children 0.10±0.01 ng/ml and C 0.03±0.002 ng/ml] and the dissociation constant KD1 [GHd-children (0.48±0.05)×10−9M and C (0.19±0.01)×10−9M] for high affinitylow capacity sites were significantly increased in erythrocytes from GHd-children, while neither receptor concentrations (R2) nor the dissociation constant (KD2) for low affinity-high capacity sites proved to be altered. These events were accompanied by a normal sensitivity to insulin as well as glucose tolerance in the GHd-group. The meaning of the increased insulin binding with normal insulin sensitivity in GH-deficiency is discussed.
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August 01 1991
Increased insulin receptor binding in erythrocytes from growth hormone-deficient children
N. Dávila;
N. Dávila
1Servicio de Endocrinología, Clínica Puerta de Hierro, Universidad Autónoma de Madrid, 28040 Madrid, Spain
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B. Barceló;
B. Barceló
1Servicio de Endocrinología, Clínica Puerta de Hierro, Universidad Autónoma de Madrid, 28040 Madrid, Spain
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M. C. Carranza;
M. C. Carranza
2Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Universidad Complutense, 28040 Madrid, Spain
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C. Calle
C. Calle
2Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Universidad Complutense, 28040 Madrid, Spain
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Publisher: Portland Press Ltd
Received:
March 28 1991
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1991 Plenum Publishing Corporation
1991
Biosci Rep (1991) 11 (4): 195–201.
Article history
Received:
March 28 1991
Citation
N. Dávila, B. Barceló, M. C. Carranza, C. Calle; Increased insulin receptor binding in erythrocytes from growth hormone-deficient children. Biosci Rep 1 August 1991; 11 (4): 195–201. doi: https://doi.org/10.1007/BF01136853
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