We studied the carbohydrate recognition systems of hepatocytes isolated from 16-day-old embryos, 19-day-old embryos and chicks within 24 h of hatching. We localized and quantified at the ultrastructural level the binding sites for glycoproteins exposing terminal N-acetylglucosamine (GlcNAc), mannose and N-acetylgalactosamine (GalNAc) residues by means of protein-gold complexes. Binding sites specific for GlcNAc and mannose residues are present on hepatocytes from embryos and chicks. On the contrary GalNAc specific binding sites are exclusively observed on cells from 16-day-old embryos. The number and distribution of gold particles on hepatocyte cell surfaces depend on the binding sites and the age considered. We describe a modulation in the number of GlcNAc, and mannose specific receptors present on the cell surface between the embryonal stage and neonatal life.
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Research Article|
October 01 1991
Chick hepatic lectins: An electron microscopic study on isolated hepatocytes during development
L. Falasca;
L. Falasca
1Department of Biology, University of Rome “Tor Vergata”, via E. Carnevale, 00173 Roma, Italy
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P. Mattioli;
P. Mattioli
1Department of Biology, University of Rome “Tor Vergata”, via E. Carnevale, 00173 Roma, Italy
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L. Dini
L. Dini
1Department of Biology, University of Rome “Tor Vergata”, via E. Carnevale, 00173 Roma, Italy
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Publisher: Portland Press Ltd
Received:
August 06 1991
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1991 Plenum Publishing Corporation
1991
Biosci Rep (1991) 11 (5): 257–264.
Article history
Received:
August 06 1991
Citation
L. Falasca, P. Mattioli, L. Dini; Chick hepatic lectins: An electron microscopic study on isolated hepatocytes during development. Biosci Rep 1 October 1991; 11 (5): 257–264. doi: https://doi.org/10.1007/BF01127501
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