Rat islets express a pertussis toxin sensitive G-protein involved in receptor-mediated inhibition of insulin secretion. This has been assumed previously to represent “Gi” which couples inhibitory receptors to adenylate cyclase. Incubation of islet G-proteins with32P-NAD and pertussis toxin resulted in the labelling of a band of molecular weight 40,000. This band was very broad and did not allow resolution of individual components. Incubation of the radiolabelled proteins with an anti-Go antiserum resulted in specific immunoprecipitation of a32P-labelled band. These results demonstrate that the complement of pertussis toxin sensitive G-proteins in rat islets includes Go.
Immunoprecipitation of a pertussis toxin substrate of the Go family from rat islets of Langerhans
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Nicholas S. Berrow, Roger D. Hurst, Susan L. F. Chan, Noel G. Morgan; Immunoprecipitation of a pertussis toxin substrate of the Go family from rat islets of Langerhans. Biosci Rep 1 April 1992; 12 (2): 95–100. doi: https://doi.org/10.1007/BF02351213
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